Abstract
Soils sampled from different locations of China were used to manipulate soil microbial diversity and to assess the effect of the diversity of the soil nitrifying community on the recovery of the soil nitrification to metal stress (zinc). Ten treatments were either or not amended with ZnCl2. Subsequently, a spike-on-spike assay was set up to test for the tolerance of soil nitrification to zinc (Zn) and copper (Cu). Initially, Zn amendment completely inhibited nitrification. After a year of Zn exposure, recovery of the potential nitrification rate in Zn-amended soils ranged from 28 to 126 % of the potential nitrification rate in the corresponding Zn-nonamended soils. This recovery was strongly related to the potential nitrification rate before Zn amendment and soil pH. Increased Zn tolerance of the soil nitrification was consistently observed in response to corresponding soil contamination. Co-tolerance to Cu was obtained in all 1,000-mg kg−1 Zn-amended soils. This tolerance was also strongly related to the potential nitrification rate before Zn amendment and soil pH. Our data indicate that inherently microbial activity can be a significant factor for the recovery of soil functioning derived from metal contamination.
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References
Bolan NS, Adriano DC, Mahimairaja S (2004) Distribution and bio-availability of trace elements in livestock and poultry manure by-products. Crit Rev Environ Sci Technol 34:291–338
Broos K, Mertens J, Smolders E (2005) Toxicity of heavy metals in soil assessed with various soil microbial and plant growth assays: a comparative study. Environ Toxicol Chem 24:634–640
Broos K, Warne MSJ, Heemsbergen DA et al (2007) Soil factors controlling the toxicity of copper and zinc to microbial processes in Australian soils. Environ Toxicol Chem 26:583–590
ISO 15685 (2004) Soil quality—determination of potential nitrification and inhibition of nitrification—rapid test by ammonium oxidation. International Organization for Standardization, Geneva
Kaitantzian A, Kelepertzis E, Kelepertsis A (2013) Evaluation of the sources of contamination in the suburban area of Koropi–Markopoulo, Athens, Greece. Bull Environ Contam Toxicol 91:23–28
Lopes C, Herva M, Franco-Uria A, Roca E (2011) Inventory of heavy metal content in organic waste applied as fertilizer in agriculture: evaluating the risk of transfer into the food chain. Environ Sci Pollut Res 18:918–939
Mertens J, Springael D, De Troyer I et al (2006) Long-term exposure to elevated zinc concentrations induced structural changes and zinc tolerance of the nitrifying community in soil. Environ Microbiol 8:2170–2178
Mertens J, Broos K, Wakelin SA et al (2009) Bacteria, not archaea, restore nitrification in a zinc-contaminated soil. ISME J 3:916–923
Nicholson FA, Smith SR, Alloway BJ et al (2003) An inventory of heavy metals inputs to agricultural soils in England and Wales. Sci Total Environ 311:205–219
Nicol GW, Leininger S, Schleper C, Prosser JI (2008) The influence of soil pH on the diversity, abundance and transcriptional activity of ammonia oxidising archaea and bacteria. Environ Microbiol 10:2966–2978
Ruyters S, Mertens J, Springael D, Smolders E (2010) Stimulated activity of the soil nitrifying community accelerates community adaptation to Zn stress. Soil Biol Biochem 42:766–772
Ruyters S, Mertens J, Springael D (2012) Co-tolerance to zinc and copper of the soil nitrifying community and its relationship with the community structure. Soil Biol Biochem 44(1):75–80
Ruyters S, Springael D, Smolders E (2013) Recovery of soil ammonia oxidation after long-term zinc exposure is not related to the richness of the bacterial nitrifying community. Microbial Ecol 66(3):312–321
Sheppard SC, Long JM, Sanipelli B (2010) Verification of radionuclide transfer factors to domestic-animal food products, using indigenous elements and with emphasis on iodine. J Environ Radiol 101:895–901
Smolders E, Brans K, Coppens F, Merckx R (2001) Potential nitrification rate as a tool for screening toxicity in metal-contaminated soils. Environ Toxicol Chem 20:2469–2474
Wei FS, Chen JS, Wu YY, Zheng CJ (1991) Elemental background values in Chinese soils. Environ Sci 12:12–19 (in Chinese)
Zhang LM, Offre PR, He JZ, Verhamme DT et al (2010) Autotrophic ammonia oxidation by soil thaumarchaea. Proc Natl Acad Sci U S A 107:17240–17245
Acknowledgments
The study was supported by the National Natural Science Foundation of China (NSFC) (No. 41001145), Projects of International Cooperation and Exchanges NSFC (41210104050), the international visiting project of Young Teachers in universities of Shandong Province, China, and the Foundation of Shandong University of Technology (No. 4072).
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Liu, A., Fang, D., Wang, C. et al. Recovery of soil nitrification after long-term zinc exposure and its co-tolerance to Cu in different soils. Environ Sci Pollut Res 22, 314–319 (2015). https://doi.org/10.1007/s11356-014-3338-1
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DOI: https://doi.org/10.1007/s11356-014-3338-1