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Isolation and characterization of two novel strains capable of using cyclohexane as carbon source

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Abstract

Two strains capable of degrading cyclohexane were isolated from the soil and sludge of the wastewater treatment plant of the University of Stuttgart and a biotrickling filter system. The strains were classified as gram negative and identified as Acidovorax sp. CHX100 and Chelatococcus sp. CHX1100. Both strains have demonstrated the capability to degrade cycloalkanes (C5–C8), while only strain CHX1100 used as well short linear n-alkanes (C5–C8) as the sole source of carbon and energy. The growth of Acidovorax sp. CHX100 using cyclohexane was much faster compared to Chelatococcus sp. CHX1100. Degenerated primers were optimized from a set sequences of cyclohexanol dehydrogenase genes (chnA) as well as cyclohexanone monooxygenases (chnB) and used to amplify the gene cluster, which encodes the conversion of cyclohexanol to caprolactone. Phylogenetic analysis has indicated that the two gene clusters belong to different groups. The cyclohexane monooxygenase-induced activity which oxidizes also indole to 5-hydroxyindole has indicated the presence of a CYP-type system monooxygenase involved in the transformation of cyclohexane to cyclohexanol.

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Acknowledgements

We thank Prof. Dr. Andreas Schmidt, PD Dr. Katja Bühler and Rohan Karande from the Laboratory of Chemical Biotechnology, TU Dortmund University, for sharing their expertise in the field of biodegradation process.

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  1. Institute for Sanitary Engineering, Water Quality and Solid Waste Management, University of Stuttgart, Bandtäle 2, 70569, Stuttgart, Germany

    Diego Salamanca & Karl-Heinrich Engesser

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  1. Diego Salamanca
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  2. Karl-Heinrich Engesser
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Correspondence to Diego Salamanca.

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Responsible editor: Robert Duran

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Salamanca, D., Engesser, KH. Isolation and characterization of two novel strains capable of using cyclohexane as carbon source. Environ Sci Pollut Res 21, 12757–12766 (2014). https://doi.org/10.1007/s11356-014-3206-z

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  • DOI: https://doi.org/10.1007/s11356-014-3206-z

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