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Characterization of endocrine disruptors from a complex matrix using estrogen receptor affinity columns and high performance liquid chromatography–high resolution mass spectrometry

  • Ecotoxicology and Environmental Toxicology : new concepts, new tools
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Abstract

Complex mixtures of contaminants with potential adverse effects on human health and wildlife are found in the environment and in the food chain. These mixtures include numerous anthropogenic compounds of various origins and structures, which may behave as endocrine disruptors. Mixture’s complexity is further enhanced by biotic and abiotic transformations. It is therefore necessary to develop new strategies allowing the identification of the structure of known, as well as unknown, nuclear receptor (NR) ligands present in complex matrices. We explored the possibility to use NR-based affinity columns to characterize the presence of bioactive molecules in environmental complex mixtures. Estrogen receptor α (ERα)-based affinity columns were used to trap and purify estrogenic substances present in surface sediment samples collected in a French river under mixed anthropogenic pressure. We combined biological, biochemical and analytical approaches to characterize the structure of ligands retained on columns and demonstrate the presence of known active molecules such as bisphenol A and octylphenol, but also of unexpected ERα ligands (n-butylparaben, hydroxyl-methyl-benzofuranone). High resolution mass spectrometry results demonstrate that ERα affinity columns can be used for the isolation, purification and identification of known as well as unknown estrogenic contaminants present in complex matrices.

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Acknowledgements

The research was supported by the «Ministère de l'Environnement, du Développement Durable et de la Mer» (MEDDM), « ECOPI » project, Grant P189. The authors want to thank Dr M. Tremblay-Franco for her help regarding statistical analyses and the reviewers for improving the quality of this manuscript.

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Authors and Affiliations

  1. INRA, UMR 1331 TOXALIM (Research Center in Food Toxicology), Xenobiotic Metabolism Team (MeX), 180 Chemin de Tournefeuille, 31027, Toulouse, France

    Adeline Jondeau-Cabaton, Amélie Soucasse & Daniel Zalko

  2. INRA, UMR 1331 TOXALIM (Research Center in Food Toxicology), MetaToul-AXIOM, 180 Chemin de Tournefeuille, 31027, Toulouse, France

    Emilien L. Jamin, Isabelle Jouanin & Laurent Debrauwer

  3. INERIS, Unité Ecotoxicologie in vitro et in vivo, Parc ALATA, BP2, 60550, Verneuil-en-Halatte, France

    Nicolas Creusot & Sélim Aït-Aïssa

  4. EPOC/LPTC-UMR 5255 CNRS Université Bordeaux 1, 33405, Talence, France

    Nicolas Creusot

  5. Plateforme CMT-Equipe Signalisation Hormonale, Environnement et Cancer IRCM, Institut de Recherche en Cancérologie de Montpellier INSERM U896-UM1-CRLC Val d’Aurelle, Parc Euromédecine, 34298, Montpellier Cedex 5, France

    Marina Grimaldi & Patrick Balaguer

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  1. Adeline Jondeau-Cabaton
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  2. Amélie Soucasse
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  3. Emilien L. Jamin
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  5. Marina Grimaldi
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  6. Isabelle Jouanin
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  7. Sélim Aït-Aïssa
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  9. Laurent Debrauwer
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  10. Daniel Zalko
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Correspondence to Daniel Zalko.

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Jondeau-Cabaton, A., Soucasse, A., Jamin, E.L. et al. Characterization of endocrine disruptors from a complex matrix using estrogen receptor affinity columns and high performance liquid chromatography–high resolution mass spectrometry. Environ Sci Pollut Res 20, 2705–2720 (2013). https://doi.org/10.1007/s11356-012-1458-z

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