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Degradation of tannic acid by cold-adapted Klebsiella sp NACASA1 and phytotoxicity assessment of tannic acid and its degradation products

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Abstract

Background, aim, and scope

The focus of the present study is to know the potential of bacterial isolate for tannic acid degradation at low temperature. Also, we tried to evaluate the suitability of phytotoxicity testing protocol for the determination of tannic acid toxicity.

Methods

Screening for tannic acid degrading bacterial strains was carried out by using microbial isolation techniques. The 16S rDNA amplicon of the isolate was used to identify the isolate. The effect of different concentrations of tannic acid and its degradation products on germination of Vigna unguiculata was evaluated. The study was carried out to determine total sugar and starch content of the used seeds and even to check the presence of α-amylase activity during seed germination.

Results

The isolated bacterium was identified as Klebsiella sp NACASA1 and it showed degradation of tannic acid in 40 (±0.85***) h at 15°C and pH 7.0. A gradual decrease in root/shoot length was observed with increasing concentration of tannic acid. There was 95.11 (±0.24**)% inhibition in α-amylase activity at 20,000 ppm tannic acid, as compared to control. No such effects were observed on germination, root–shoot length, and α-amylase activity with tannic acid degradation products.

Conclusions

The results obtained confirmed that tannic acid may act as a toxic agent in plant cells. The simple biodegradation process presented in this study was found to be effective in reducing toxicity of tannic acid. Also, it reveals the potential of soil bacterium to degrade tannic acid at low temperature.

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References

  • Archambault J, Lacki K, Duvnjak Z (1996) Conversion of catechin and tannic acid by an enzyme preparation from Trametes versicolor. Biotechnol Lett 18:771–774

    Article  CAS  Google Scholar 

  • Basaraba J (1966) Effects of vegetable tannins on glucose oxidation by various microorganisms. Can J Microbiol 12:787–794

    Article  CAS  Google Scholar 

  • Beck E, Ziegler P (1989) Biosynthesis and degradation of starch in higher plants. Annu Rev Plant Physiol Plant Mol Biol 40:95–117

    Article  CAS  Google Scholar 

  • Bhakta Dipita and Ganjewala Deepak (2009) Effect of Leaf Positions on Total Phenolics, Flavonoids and Proantho-cyanidins Content and Antioxidant Activities in Lantana Camara (L) J. Sci. Res. 1 (2),363-369

    Google Scholar 

  • Bhat T, Singh B, Sharma O (1998) Microbial degradation of tannins—a current perspective. Biodegradation 9:343–357

    Article  CAS  Google Scholar 

  • Chiacchierini E, Restuccia D, Vinci G (2004) Bioremediation of food industry effluents: recent applications of free and immobilised polyphenoloxidases. Food Sci Technol Int 10:373–382

    Article  CAS  Google Scholar 

  • Colpas F, Ono E, Rodrigues J, Passos J (2003) Effects of some phenolic compounds on soybean seed germination and on seed-borne fungi. Braz Arch Biol Technol 46:155–161

    Article  CAS  Google Scholar 

  • Deschamps A, Mahoudeau G, Conti M, Lebeault J (1980) Bacteria degrading tannic acid and related compounds. J Ferment Technol 58:93–97

    CAS  Google Scholar 

  • Deschamps A, Otuk G, Lebeault J (1983) Production of tannase and degradation of chestnut tannins by bacteria. J Ferment Technol 61:55–59

    CAS  Google Scholar 

  • Dubios M, Gilles J, Robers P, Smith F (1951) Colorimetric determination of sugar and related substances. Anal Chem 26:351–356

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Field J, Lettinga G (1992) Biodegradation of tannins. In: Sigel H (ed) Metal ions in biological systems volume 28. Degradation of environmental pollutants by microorganisms and their metalloenzymes. Marcel Dekker, New York, pp 61–97

    Google Scholar 

  • Fincher G (1989) Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu Rev Plant Physiol Plant Mol Biol 40:305–345

    Article  CAS  Google Scholar 

  • Franco A, Calheiros C, Pacheco C, De-Marco P, Manaia C, Castro P (2005) Isolation and characterization of polymeric galloyl-ester-degrading bacteria from a tannery discharge place. Microb Ecol 50:550–556

    Article  CAS  Google Scholar 

  • Fuchs G, Mohamed M, Altenschmidt U, Koch J, Lack A, Brackmann R, Lochmeyer C, Oswald B (1994) Biochemistry of anaerobic biodegradation of aromatic compounds. In: Ratledge C (ed) Biochemistry of microbial degradation. Kluwer, Dordrecht

    Google Scholar 

  • Gandhi P (1990) Microbial degradation with special reference to tannery. Doctoral thesis, Madurai Kamaraj University, Madurai, India

  • Garg S, Makkar H, Nagal K, Sharma S, Wadhwa D, Singh B (1992) Toxicological investigations into oak (Quercus incana) leaf poisoning in cattle. Vet Hum Toxicol 34:161–164

    CAS  Google Scholar 

  • Geerdink M, Loosdrecht M, Luyben K (1996) Biodegradability of diesel oil. Biodegradation 7:73–81

    Article  CAS  Google Scholar 

  • Gu L, Kelm M, Hammerstone J, Beecher G, Holden J, Haytowitz D, Prior R (2003) Screening of foods containing proanthocyanidins and their structural characterization using LC-MS/MS and thiolytic degradation. J Agric Food Chem 51:7513–7521

    Article  CAS  Google Scholar 

  • Gubler F, Kalla R, Roberts J, Jacobsen J (1995) Gibberellin-regulated expression of a myb gene in barley aleurone cells: evidence for Myb transactivation of a high-pI alpha-amylase gene promoter. Plant Cell 7:1879–1891

    Article  CAS  Google Scholar 

  • Haslam E (1966) The scope of vegetable tannin chemistry. Chemistry of vegetable tannins. Academic, London, pp 1–13

    Google Scholar 

  • Ilori M, Adebusoye S, Amund O, Oyetoran B (2007) A study of tannic acid degradation by soil bacteria. Pak J Biol Sci 10:3224–3227

    Article  CAS  Google Scholar 

  • Kapanen A, Itavaara M (2001) Ecotoxicity tests for compost applications. Ecotoxicol Environ Saf 49:1–16

    Article  CAS  Google Scholar 

  • Lee C, Russell N, White G (1995) Rapid screening for bacterial phenotypes capable of biodegrading anionic surfactants: development and validation of a microtitre plate method. Microbiol 141:2801–2810

    Article  CAS  Google Scholar 

  • Lekha P, Lonsane B (1997) Production and application of tannin acyl hydrolase: state of the art. Adv Appl Microbiol 44:215–260

    Article  CAS  Google Scholar 

  • Lowry O, Rosebrough N, Farr A, Randall R (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Marchesi J, White G, Russell N, House W (1997) Effect of river sediment on the biodegradation kinetics of surfactant and non-surfactant compounds. FEMS Microbiol Ecol 23:55–63

    Article  CAS  Google Scholar 

  • Margesin R, Schinner F (1998) Low-temperature bioremediation of a waste water contaminated with anionic surfactant and fuel oil. Appl Microbiol Biotechnol 49:482–486

    Article  CAS  Google Scholar 

  • Margesin R, Schinner F (1999) Biodegradation of organic pollutants at low temperatures. In: Margesin R, Schinner F (eds) Biotechnological applications of cold-adapted organisms. Springer, Berlin, pp 271–289

    Chapter  Google Scholar 

  • McCready R, Guggolz J, Silviera V, Owens H (1950) Determination of starch and amylase in vegetables. Anal Chem 22:1156–1158

    Article  CAS  Google Scholar 

  • Mingshu L, Kai Y, Qiang H, Dongying J (2006) Biodegradation of gallotanins and ellagitannins. J Basic Microbiol 46:68–84

    Article  Google Scholar 

  • Monier J, Lindow S (2005) Aggregates of resident bacteria facilitate survival of immigrant bacteria on leaf surfaces. Microb Ecol 49:343–352

    Article  Google Scholar 

  • Moreland D, Novitzky W (1987) Effects of phenolic acids, coumarins, and flavonoids on isolated chloroplasts and mitochondria. In: Waller GR (ed) Allelochemicals: role in agriculture and forestry. American Chemical Society, Washington, pp 247–261

    Chapter  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  Google Scholar 

  • Scalbert A (1991) Antimicrobial properties of tannins. Phytochem 30:3875–3883

    Article  CAS  Google Scholar 

  • Sengupta S, Jana M, Sengupta D, Naskar A (2000) A note on the estimation of microbial glycosidase activities by dinitrosalicylic acid reagent. Appl Microbiol Biotechnol 53:732–735

    Article  CAS  Google Scholar 

  • Somasegaram P, Hoben H (1985) Methods in legume Rhizobium technology. NifTAL, Paia Maui

    Google Scholar 

  • Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci USA 101:11030–11035

    Article  CAS  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  Google Scholar 

  • Timmis K, Pieper D (1999) Bacteria designed for bioremediation. Trends Biotechnol 17:201–204

    Article  CAS  Google Scholar 

  • Tiquia S (2010) Reduction of compost phytotoxicity during the process of decomposition. Chemosphere 79:506–512

    Article  CAS  Google Scholar 

  • Yamamoto Y, Fujii Y (1997) Exudation of allelopathic compound from plant roots of sweet vernalgrass (Anthoxanthum odoratum). J Weed Sci Technol 42:31–35

    Article  CAS  Google Scholar 

  • Zucconi F, Forte M, Monaco A, De-Bertoldi M (1981a) Biological evaluation of compost maturity. BioCycle 22:27–29

    CAS  Google Scholar 

  • Zucconi F, Pera A, Forte M, De-Bertoldi M (1981b) Evaluating toxicity of immature compost. BioCycle 22:54–57

    Google Scholar 

Download references

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Author notes

  1. Umesh Jadhav

    Present address: Department of Power Mechanical Engineering, National Tsing Hua University, No. 101, Sec. 2, Kuang Fu Rd, 30013, Hsinchu, Taiwan, Republic of China

Authors and Affiliations

  1. P.G. Department of Biochemistry, N.A.C. & S. College, University of Pune, Ahmednagar, 414 001, India

    Sudhir Kadu & Nilesh Thokal

  2. Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, 431004, India

    Manohar Padul

  3. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune, India

    Vishal Dawkar

  4. Mass Spectrometry and Proteomics Group, Organic Chemistry Division, National Chemical Laboratory, Pune, India

    Ashok Chougale

  5. Department of Botany, Hutatma Rajguru Mahavidayala, University of Pune, Rajgurunagar, India

    Abhay Salve

  6. P.G. Department of Biotechnology, N.A.C. & S. College, University of Pune, Ahmednagar, 414 001, India

    Manoj Patil

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  1. Umesh Jadhav
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Correspondence to Umesh Jadhav.

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Responsible editor: Elena Maestri

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Jadhav, U., Kadu, S., Thokal, N. et al. Degradation of tannic acid by cold-adapted Klebsiella sp NACASA1 and phytotoxicity assessment of tannic acid and its degradation products. Environ Sci Pollut Res 18, 1129–1138 (2011). https://doi.org/10.1007/s11356-011-0468-6

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