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Measurement of levels of lung HIF-1α protein in response to tapering for 14- and 21-day with nigella sativa supplementation in maturing rat, with histological study

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Abstract

The aim of this study was to evaluate lung histology and the levels of HIF-1α protein in response to the reduction in training volume for 14 and 21 days with Nigella sativa (NS) supplementation. 60 three-week-old male Wistar rats (68 ± 9 g) were divided into 6 groups after 6 weeks of incremental interval training (IIT); IIT control and IIT groups, 2 groups of 14-day taper (with and without NS) and, similarly, 2 groups of 21-day taper. After 6 weeks, NS seed ethanol extract was fed daily by gavages to NS groups at 500 mg/kg. Finally, animals were euthanized and their lungs were removed, weighed and fixed in 10 % formalin. By routine and standard paraffin embedding, lung samples were sectioned in 5 μm and stained with H&E and studied histologically. The level of HIF-1α was measured by ELISA kit. The results showed that the administration of NS and 21-day taper in the IIT exposed rats caused significant difference in lung weight and lung HIF-1α (p = 0.001, p = 0.001). But the interaction between groups and taper periods was not confirmed (p = 0.530). Lung histology in 21-day taper with NS was better than the other groups. The results showed that after IIT, 21-day reduction in training volume and the use of new strategies such as NS supplementation in this period can reduce HIF-1α higher amounts in the lungs, which may probably be due to the anti-inflammatory effects of the NS supplement. These agents can improve respiratory capacity for competition or severe activity after taper period.

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Abbreviations

NS:

Nigella sativa

IIT:

Incremental interval training

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Acknowledgments

We are grateful to Mehdi Hedayati and Gholamreza Hamidian for their analytical help. We also acknowledge the Department of Exercise Physiology at the University of Mazandaran for their contributions.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This study was conducted according to the Declaration of Helsinki guidelines and approved by the Ethical Committee of the University of Mazandaran.

Informed consent

Informed consent was obtained from all subjects for being included in the study.

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Authors and Affiliations

  1. Department of Cellular and Molecular Exercise Physiology, Faculty of Physical Education and Sport Sciences, Urmia University, Urmia, Iran

    Ehsan Arabzadeh

  2. Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran

    Shadmehr Mirdar & Zohreh Fathi

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  1. Ehsan Arabzadeh
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  2. Shadmehr Mirdar
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  3. Zohreh Fathi
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Correspondence to Ehsan Arabzadeh.

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Arabzadeh, E., Mirdar, S. & Fathi, Z. Measurement of levels of lung HIF-1α protein in response to tapering for 14- and 21-day with nigella sativa supplementation in maturing rat, with histological study. Sport Sci Health 11, 195–202 (2015). https://doi.org/10.1007/s11332-015-0223-3

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  • DOI: https://doi.org/10.1007/s11332-015-0223-3

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