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Diurnal variation of circulating microvesicles is associated with the severity of obstructive sleep apnoea

  • Sleep Breathing Physiology and Disorders • Original Article
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Abstract

Purpose

Microvesicles (MVs) have been implicated in the pathomechanism of obstructive sleep apnoea (OSA); however, the results are inconsistent, possibly due to an unrevealed temporal variation in circulating MV levels. We aimed to investigate the diurnal changes of MV fractions in OSA.

Methods

Peripheral blood was taken from 18 patients with OSA and 9 healthy subjects at different time points (11:00, 17:00, 21:00, 01:30 and 06:00). Samplings were repeated in nine OSA patients after 2 months of continuous positive airway pressure (CPAP) therapy. CD41+, CD62P+, glycophorin A+ and Annexin V+ MVs were determined with flow cytometry. Areas under the MV concentrations-time curves (AUC) were calculated and correlated with the severity of OSA.

Results

A significant diurnal variability of plasma CD41+ and Annexin V+ MVs was observed only in OSA with a marked peak at 17:00. There was a direct correlation between CD41+ MV AUCs and the severity of OSA. CPAP treatment reduced diurnal variability in both CD41+ and Annexin V+ MV levels.

Conclusions

The relationship between the diurnal variability of CD41+ MVs and disease severity as well as the effect of CPAP treatment on MV levels support the role of MVs in the pathophysiology of OSA. More importantly, considering the significant diurnal variation in circulating MV levels, introduction of strict protocols for blood sampling is required for MV measurements.

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Acknowledgements

The study was supported by Hungarian Respiratory Society (grants to Andras Bikov and Zsolt I. Komlosi) as well as Hungarian Scientific Research Fund (OTKA 68758, 109008). The authors are also grateful to Elektro-Oxigén Inc. for providing the polysomnographic devices. This publication was supported by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences to Andras Bikov. The authors are extremely grateful to Mrs. Monika Banlaky for her technical assistance with PSG.

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Authors and Affiliations

  1. Department of Pulmonology, Semmelweis University, Diós árok 1/C, Budapest, 1125, Hungary

    András Bikov, László Kunos, Zsófia Lázár, Adrián Kis, Gábor Horváth, György Losonczy & Zsolt István Komlósi

  2. Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvárad tér 4, Budapest, 1089, Hungary

    Éva Pállinger

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  1. András Bikov
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  2. László Kunos
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  4. Zsófia Lázár
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  7. György Losonczy
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  8. Zsolt István Komlósi
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Correspondence to Zsolt István Komlósi.

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Funding

Hungarian Respiratory Society (grants to Andras Bikov and Zsolt I. Komlosi) as well as Hungarian Scientific Research Fund (OTKA 68758, 109008) provided financial support in the form of funding to buy materials and reagents for experiments. The sponsor had no role in the design or conduct of this research.

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Bikov, A., Kunos, L., Pállinger, É. et al. Diurnal variation of circulating microvesicles is associated with the severity of obstructive sleep apnoea. Sleep Breath 21, 595–600 (2017). https://doi.org/10.1007/s11325-017-1464-y

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  • DOI: https://doi.org/10.1007/s11325-017-1464-y

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