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[99mTc]Annexin V-128 SPECT Monitoring of Splenic and Disseminated Listeriosis in Mice: a Model of Imaging Sepsis

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Abstract

Purpose

Here, we evaluate [99mTc]annexin V-128, an in vivo marker of apoptosis, for single photon emission computed tomography (SPECT) imaging of localization and antibiotic treatment of disseminated bacterial infection, using a well-described mouse model that employs bioluminescent Listeria monocytogenes.

Procedures

Sixteen groups of five mice in six separate experiments were infected with bioluminescent Listeria, and in vivo bioluminescence imaging (BLI) was performed each day, to assess the location and extent of infection and response to antibiotics. [99mTc]annexin V-128 was then injected for SPECT imaging, and the two sets of images were correlated and validated.

Results

Signals from BLI and [99mTc]annexin V-128 SPECT co-localized within the spleen and other organs including bone marrow, intestine, nasopharynx, and brain. Decreases in [99mTc]annexin V-128 uptake and BLI signal within the spleen directly reflected the reduction of bacterial infection by ampicillin treatment.

Conclusions

Tc-99m-Annexin V-128 uptake as observed by SPECT allowed for the detection of systemic listeriosis and ampicillin treatment in mice. [99mTc]annexin V-128 should be further explored for the assessment of bacterial spread and antibiotic efficacy in patients with disseminated bacterial infection.

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Acknowledgments

We gratefully acknowledge Pauline Chu for histology, Timothy Doyle and the SCI3 Facility at Stanford University for help with imaging, and Valeria Muzio for critical reading of the manuscript.

Conflict of Interest

We declare no potential conflict of interest with regard to this manuscript or the studies therein described. This work was funded in part by the Fund for Excellence in Pediatric Research from the Chambers Family Foundation.

Author information

Author notes

  1. Zoia Levashova

    Present address: Igenica Inc., 863 Mitten Rd., Suite 102, Burlingame, CA, 94010, USA

Authors and Affiliations

  1. Department of Pediatrics, Stanford University School of Medicine, E150 Clark Center MC 5427, Stanford, CA, 94305, USA

    Jonathan W. Hardy, Tobi L. Schmidt & Christopher H. Contag

  2. Divisions of Pediatric Radiology & Nuclear Medicine, Department of Radiology, Lucile Packard Children’s Hospital, Stanford, CA, 94305, USA

    Zoia Levashova, Christopher H. Contag & Francis G. Blankenberg

  3. Department of Microbiology and Immunology, Stanford University School of Medicine, E150 Clark Center MC 5427, Stanford, CA, 94305, USA

    Christopher H. Contag

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  1. Jonathan W. Hardy
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  2. Zoia Levashova
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  4. Christopher H. Contag
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  5. Francis G. Blankenberg
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Correspondence to Jonathan W. Hardy.

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Hardy, J.W., Levashova, Z., Schmidt, T.L. et al. [99mTc]Annexin V-128 SPECT Monitoring of Splenic and Disseminated Listeriosis in Mice: a Model of Imaging Sepsis. Mol Imaging Biol 17, 345–354 (2015). https://doi.org/10.1007/s11307-014-0804-6

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  • DOI: https://doi.org/10.1007/s11307-014-0804-6

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