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Transcriptome analysis of Ulmus pumila ‘Jinye’ responses to different shading involved in chlorophyll metabolism

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Abstract

A variety of colorful mutations exist in nature. However, shade stress will affect the color of ornamental plants. Ulmus pumila ‘Jinye’ is a cultivated species of elm with a yellow leaf color, which will become green under shade stress. In this paper, ‘Jinye’ samples were treated with different degrees of shade by using shading net to investigate the mechanism of greening of the yellow leaves through phenotype observation and high-throughput sequencing. The height of plants rose in slight shade stress then fell in heavy shade stress. The content of chlorophyll and carotenoid increased with the increase of shade degree, and the leaf size increased as well. On the other hand, the net photosynthesis capacity declined. About 32.17 Gb of clean data were obtained after the completion of sequencing. A total of 33,023 unigenes with annotated information were obtained after comparing ‘Jinye’ with other species. The number of differentially expressed genes increased, and the proportion of upregulated genes decreased with increasing shade. The differentially expressed gene (DEG) analysis showed that the porphyrin and chlorophyll metabolism of Ulmus pumila ‘Jinye’ is affected by light. This may be related to genes associated with pigment synthesis. This first sequencing result of U. pumila will provide a basis for further research into this species.

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Acknowledgements

This research was supported by the National Natural Science Foundation of China (No:31370664) and the Graduate Innovation Funding of Hebei (No:1099009).

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Author notes

  1. Shuang Zhang and Lihui Zuo contributed equally to this study.

Authors and Affiliations

  1. Institute of Forest Biotechnology, Forestry College, Agricultural University of Hebei, 071000, Baoding, People’s Republic of China

    Shuang Zhang, Lihui Zuo, Jun Zhang, Panfei Chen, Jinmao Wang & Minsheng Yang

  2. Hebei Key Laboratory for Tree Genetic Resources and Forest Protection, 071000, Baoding, People’s Republic of China

    Shuang Zhang, Lihui Zuo, Jun Zhang, Panfei Chen, Jinmao Wang & Minsheng Yang

Authors
  1. Shuang Zhang
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  2. Lihui Zuo
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  3. Jun Zhang
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  4. Panfei Chen
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  5. Jinmao Wang
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  6. Minsheng Yang
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Corresponding authors

Correspondence to Jinmao Wang or Minsheng Yang.

Additional information

Communicated by A. Brunner

Data archiving statement

Sequencing data are available through BioProject PRJNA349076. Four samples were run on an Illumina Hiseq2500 at Beijing Biomarker Technology, China, on August 2015. The samples were control (SAMN05915700), T1 (SAMN05915701), T2 (SAMN05915704), and T3 (SAMN05915703) leaves.

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Zhang, S., Zuo, L., Zhang, J. et al. Transcriptome analysis of Ulmus pumila ‘Jinye’ responses to different shading involved in chlorophyll metabolism. Tree Genetics & Genomes 13, 64 (2017). https://doi.org/10.1007/s11295-017-1139-7

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  • DOI: https://doi.org/10.1007/s11295-017-1139-7

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