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QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny

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Abstract

Brown rot (BR) caused by Monilinia spp. leads to significant post-harvest losses in stone fruit production, especially peach. Previous genetic analyses in peach progenies suggested that BR resistance segregates as a quantitative trait. In order to uncover genomic regions associated with this trait and identify molecular markers for assisted selection (MAS) in peach, an F1 progeny from the cross “Contender” (C, resistant) × “Elegant Lady” (EL, susceptible) was chosen for quantitative trait loci (QTL) analysis. Over two phenotyping seasons, skin (SK) and flesh (FL) artificial infections were performed on fruits using a Monilinia fructigena isolate. For each treatment, infection frequency (if) and average rot diameter (rd) were scored. Significant seasonal and intertrait correlations were found. Maturity date (MD) was significantly correlated with disease impact. Sixty-three simple sequence repeats (SSRs) plus 26 single-nucleotide polymorphism (SNP) markers were used to genotype the C × EL population and to construct a linkage map. C × EL map included the eight Prunus linkage groups (LG), spanning 572.92 cM, with an average interval distance of 6.9 cM, covering 78.73 % of the peach genome (V1.0). Multiple QTL mapping analysis including MD trait as covariate uncovered three genomic regions associated with BR resistance in the two phenotyping seasons: one containing QTLs for SK resistance traits near M1a (LG C × EL-2, R 2 = 13.1–31.5 %) and EPPISF032 (LG C × EL-4, R 2 = 11–14 %) and the others containing QTLs for FL resistance, near markers SNP_IGA_320761 and SNP_IGA_321601 (LG3, R 2 = 3.0–11.0 %). These results suggest that in the C × EL F1 progeny, skin resistance to fungal penetration and flesh resistance to rot spread are distinguishable mechanisms constituting BR resistance trait, associated with different genomic regions. Discovered QTLs and their associated markers could assist selection of new cultivars with enhanced resistance to Monilinia spp. in fruit.

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Acknowledgments

This work was supported by an Italian grant to DB funded by private and public agencies “MAS.PES: apricot and peach breeding by molecular-assisted selection.” Thanks to the Executive Committee of Fruit Breedomics European Project (FP7-265582) and Fondazione Edmund Mach, who allowed the use and provided genotypic data of 26 SNPs included in this study. RP was supported by MIUR FIRB grant RBIP06CTBR. The authors wish to thank M Pesenti, S Femia, A Masini, M Lasala, A Arioli, L Nazzari, and E Quattrini for technical assistance in field and lab operations, as well as Dr. S Biffani for the advice on statistical analyses and Prof. F Faoro for the advice on plant pathology issues.

Author contributions

IP constructed the genetic map, performed and coordinated phenotyping, conducted statistical and QTL analysis and drafted and corrected the manuscript. DB assessed the potential of the plant material, developed the population, helped to draft the manuscript, managed and coordinated the study. IE and RP participated in marker analysis and helped to draft the manuscript. AC helped in phenotyping, QTL analyses, and helped in the revision of the manuscript. LR managed and coordinated the study and helped to draft the manuscript. AV managed and coordinated the study, participated in its design. All authors read and approved the final manuscript.

Data archiving statement

C × EL linkage map and QTL positions will be submitted on Genome Database for Rosaceae (www.rosaceae.org).

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Authors and Affiliations

  1. Dipartimento di Scienze Agrarie e Ambientali, Produzione, Territorio, Agroenergia, Università degli Studi di Milano, Via Celoria 2, 20133, Milan, Italy

    Igor Pacheco, Daniele Bassi, Angelo Ciacciulli & Laura Rossini

  2. Parco Tecnologico Padano, Via Einstein, Loc. Cascina Codazza, 26900, Lodi, Italy

    Iban Eduardo, Raul Pirona, Laura Rossini & Alberto Vecchietti

  3. Consiglio Nazionale per la Ricerca, Institute of Agricultural Biology and Biotechnology, Via Bassini 15, 20133, Milan, Italy

    Raul Pirona

  4. Departament de Genètica Vegetal, Centre de Recerca en Agrigenòmica CSIC-IRTA-UAB (CRAG), Campus UAB, 08193 Bellaterra, Barcelona, Spain

    Iban Eduardo

  5. Monsanto Vegetable Seeds Division, P.O. Box 1050, 2660 BB, Bergschenhoek, The Netherlands

    Alberto Vecchietti

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  1. Igor Pacheco
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  2. Daniele Bassi
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  3. Iban Eduardo
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  4. Angelo Ciacciulli
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  6. Laura Rossini
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  7. Alberto Vecchietti
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Corresponding author

Correspondence to Igor Pacheco.

Additional information

Communicated by E. Dirlewanger

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Pacheco, I., Bassi, D., Eduardo, I. et al. QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny. Tree Genetics & Genomes 10, 1223–1242 (2014). https://doi.org/10.1007/s11295-014-0756-7

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