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Bioethanol production from steam-exploded rice husk by recombinant Escherichia coli KO11

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Abstract

Rice husk is one of the most abundant types of lignocellulosic biomass. Because of its significant amount of sugars, such as cellulose and hemicellulose, it can be used for the production of biofuels such as bioethanol. However, the complex structure of lignocellulosic biomass, consisting of cellulose, hemicellulose and lignin, is resistant to degradation, which limits biomass utilization for ethanol production. The protection of cellulose by lignin contributes to the recalcitrance of lignocelluloses to hydrolysis. Therefore, we conducted steam-explosion treatment as pretreatment of rice husk. However, recombinant Escherichia coli KO11 did not ferment the reducing sugar solution obtained by enzymatic saccharification of steam-exploded rice husk. When the steam-exploded rice husk was washed with hot water to remove inhibitory substances and M9 medium (without glucose) was used as a fermentation medium, E. coli KO11 completely fermented the reducing sugar solution obtained by enzymatic saccharification of hot water washing-treated steam-exploded rice husk to ethanol. We report here the efficient production of bioethanol using steam-exploded rice husk.

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Acknowledgements

This work was supported in part by a Grant for the Program for the Strategic Research Foundation at Private Universities S1101017 since April 2011 (to NS).

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Authors and Affiliations

  1. Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Ora-gun, Gunma, 374-0193, Japan

    Takamitsu Tabata, Yusuke Yoshiba, Tomonori Takashina & Norio Shimizu

  2. Frontier Foods Co. Ltd., 3-3-2 Kandaogawa-machi, Chiyoda-ku, Tokyo, 101-0052, Japan

    Kazuo Hieda

  3. Bio-Nano Electronics Research Center, Toyo University, 2100 Kujirai, Kawagoe-shi, Saitama, 350-8585, Japan

    Norio Shimizu

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  1. Takamitsu Tabata
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  2. Yusuke Yoshiba
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  3. Tomonori Takashina
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  4. Kazuo Hieda
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  5. Norio Shimizu
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Correspondence to Norio Shimizu.

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Tabata, T., Yoshiba, Y., Takashina, T. et al. Bioethanol production from steam-exploded rice husk by recombinant Escherichia coli KO11. World J Microbiol Biotechnol 33, 47 (2017). https://doi.org/10.1007/s11274-017-2221-x

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  • DOI: https://doi.org/10.1007/s11274-017-2221-x

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