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Rapid detection of Salmonella enterica serovars by multiplex PCR

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Abstract

A multiplex PCR based assay was developed for the identification of the genus Salmonella. Five sets of primers from different genomic sequences such as fimA, himA, hns, invA and hto genes were selected for the identification of serogroups of Salmonella enterica such as S. Typhi, S. ParatyphiA, S. Typhimurium, S. Enteritidis and S. Weltevreden. The selected primers amplified products with the sizes of 85, 123, 152, 275 and 496 bp, respectively, for the genus Salmonella. This assay was found to be highly sensitive, as it could detect 5 cells of Salmonella and 1,000 fg of genomic DNA. Amplification of DNA extracted from other genera viz. V. cholerae and E. coli yielded negative results. This assay provides specific and reliable results and allows for the cost–effective detection of Salmonella in one reaction tube in mixed bacterial communities.

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Acknowledgments

The financial assistance extended by the Dept. of Biotechnology, Govt. of India, New Delhi, India for conducting this project work is gratefully acknowledged. Authors wish to thank the Dean, Fisheries College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tuticorin, India for having provided necessary support for carrying out this work.

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Authors and Affiliations

  1. Department of Fish Processing Technology, Fisheries College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Tuticorin, 628 008, India

    G. Jeyasekaran, K. Thirumalai Raj, R. Jeya Shakila, A. Jemila Thangarani, D. Sukumar & V. Abdul Kadhar Jailani

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  1. G. Jeyasekaran
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  2. K. Thirumalai Raj
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  3. R. Jeya Shakila
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  4. A. Jemila Thangarani
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  5. D. Sukumar
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  6. V. Abdul Kadhar Jailani
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Correspondence to G. Jeyasekaran.

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Jeyasekaran, G., Raj, K.T., Shakila, R.J. et al. Rapid detection of Salmonella enterica serovars by multiplex PCR. World J Microbiol Biotechnol 27, 953–959 (2011). https://doi.org/10.1007/s11274-010-0538-9

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  • DOI: https://doi.org/10.1007/s11274-010-0538-9

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