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Endophytic fungi from a pharmaceutical plant, Camptotheca acuminata: isolation, identification and bioactivity

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Abstract

About 174 endophytic fungi were isolated from the pharmaceutical plant, Camptotheca acuminata. Of the 18 taxa obtained, non-sporulating fungi (48.9%), Alternaria (12.6%), Phomopsis (6.9%), Sporidesmium (6.3%), Paecilomyces (4.6%) and Fusarium (4.6%) were dominant. ITS rDNA assay indicated that most of the non-sporulating fungi belonged to the Pyrenomycetes and Loculoascomycetes ascomycetes or their anamorph Coelomycetes.

The results of the bioactivity test showed that 27.6% of the endophytic fungi displayed inhibition against more than one indicator microorganism. 4.0% and 2.3% of the endophytic fungi showed cytotoxicity and protease inhibition, respectively. The endophytic fungi with bioactivities were distributed in more than 12 taxa including non-sporulating fungi, which are reliable sources for bioactive agents.

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References

  • Ainsworth GC, Sparrow FK, Sussman AS (1973) The fungi, an advanced treatise. Edition Vol IV A, A taxonomic review with keys ascomycetes and fungi imperfecti. Academic Press, New York

    Google Scholar 

  • Gao lD, Hyde KD, Liew ECY (2001) Detection and taxonomic placement of endophytic fungi within frond tissues of Livistona chinensis based on rDNA sequences. Mol Phylogenet Evol 20:1–13

    Article  CAS  Google Scholar 

  • Gauthier MJ (1975) Taxonomic position and seasonal variation in marine environment of some Gram-negative antibiotic-producing bacteria. J Gen Microbiol 87:211–218

    PubMed  CAS  Google Scholar 

  • Huang LH, Kaneka T (1996) Pyrenomycetes and Loculoascomycetes as source of secondary metabolites. J Ind Microbiol 17:402–416

    Article  CAS  Google Scholar 

  • Huang YJ, Wang JF, Li GL et al (2001) Antitumor and antifungal activities in endophytic fungi isolated from pharmaceutical plants. FEMS Immunol Med Microbiol 31:163–167

    Article  PubMed  CAS  Google Scholar 

  • Liang XQ, Pan RC, Zhou GY (2003) Relationship of trypsin inhibitor in peanut seed and resistance to Aspergillus flavus invasion. Acta Agric Sin 29:295–299

    Google Scholar 

  • Mclaughlin DJ, Mclaughlin EG, Lemke PA (2001) Systematics and evolution Part A. In: Esser K, Lemke PA (eds) The mycota VII: a comprehensive treatise on fungi as experimental systems for basic and applied research. Springer

  • Mosmann F (1983) Rapid calorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Methods 65:55–63

    Article  PubMed  CAS  Google Scholar 

  • Stierle A, Strobel G, Stierle D (1993) Taxol and taxol production by Taxomyces andreanae, an endophytic fungus of pacific yew. Science 260:214–216

    Article  PubMed  CAS  ADS  Google Scholar 

  • Strobel G, Daisy B (2003) Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67:491–502

    Article  PubMed  CAS  Google Scholar 

  • Strobel G, Yang XS, Sears J et al (1996) Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana. Microbiology 142:435–440

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

We are grateful for the support of National Natural Sciences Foundation of China (30500632).

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Correspondence to Yaojian Huang.

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Lin, X., Lu, C., Huang, Y. et al. Endophytic fungi from a pharmaceutical plant, Camptotheca acuminata: isolation, identification and bioactivity. World J Microbiol Biotechnol 23, 1037–1040 (2007). https://doi.org/10.1007/s11274-006-9329-8

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  • DOI: https://doi.org/10.1007/s11274-006-9329-8

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