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Assessment of HCV genotypes in Yunnan Province of Southwest China

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Abstract

Recently, we reported that the frequency of hepatitis C virus (HCV) genotypes and subtypes has rapidly changed among intravenous drug users (IDUs) in Yunnan Province over the last 5 years; this is especially true for subtype 6a which has increased in frequency from 5 to 15%. Here, we assessed 120 HCV-positive plasma samples from the general population (GP). HCV NS5B fragments were amplified and sequenced by PCR. We identified four HCV genotypes (1, 2, 3 and 6) and seven HCV subtypes (1b, 2a, 3a, 3b, 6a, 6n, and 6k) in this population. Genotype 3 was predominant, with a distribution frequency of 0.484, followed by genotype 1 (0.283), genotype 6 (0.133) and genotype 2 (0.100). HCV subtypes 3b (frequency 0.292) and 1b (frequency 0.283) were the most common subtypes. A comparison of the current data with previous results reported for IDUs showed that the distribution frequencies of genotypes 1, 2 and 6 were significantly different between patients in the GP and IDUs (P < 0.05). Among the HCV subtypes, the distribution frequencies of 1b, 2a, 6a, and 6n were significantly different between patients in the GP and IDU groups (P < 0.05). Moreover, Phylogenetic analyses showed that HCV subtype 6a strains isolated from IDUs and the GP were intermixed and not separately clustered. HCV subtype 6a was predominant not only among IDUs but also among those in the GP in the Guangdong Province and Vietnam. However, HCV subtype 6a was predominant only among IDUs and not among those in the GP in the Yunnan and Guangxi Provinces. Our results indicate that the HCV subtype 6a could rapidly spread across China.

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Abbreviations

HCV:

Hepatitis C virus

IDUs:

Intravenous drug users

GP:

General population

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Acknowledgements

This work was supported by grants from the Wujieping Medical Foundation (320.6750.13395), National Natural Science Foundation of China (81160197) and Yunnan Provincial Science and Technology Department (2013FZ131).

Author Contributions

LS and SD conceived and designed the experiments; QL, YS, DC, and JY performed the experiments; YY and MS analysed the data; YL, SZ, and WC contributed materials; and LS wrote the paper.

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Authors and Affiliations

  1. Division for Expanded Program of Immunization of Yunnan, Center for Disease Control and Prevention, Kunming, 650011, China

    Qiongfen Li

  2. Institute of Medical Biology, Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Disease, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, 650118, China

    Yufeng Yao, Danfeng Cao, Wei Cun, Mingbo Sun, Jiankun Yu, Li Shi & Shaozhong Dong

  3. The First People’s Hospital in Yunnan Province & The Affiliated Hospital of Kunming Science and Technology University, Kunming, 650032, China

    Yunsong Shen & Yalin Li

  4. The Third People’s Hospital of Kunming, Kunming, 650041, China

    Shuqiong Zhang

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  1. Qiongfen Li
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  10. Li Shi
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Correspondence to Li Shi or Shaozhong Dong.

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Written informed consent was obtained from the patients prior to publication.

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Edited by Detlev H. Kruger.

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Li, Q., Yao, Y., Shen, Y. et al. Assessment of HCV genotypes in Yunnan Province of Southwest China. Virus Genes 53, 190–196 (2017). https://doi.org/10.1007/s11262-016-1420-0

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  • DOI: https://doi.org/10.1007/s11262-016-1420-0

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