Abstract
A novel mammalian orthoreovirus (MRV) strain was isolated from the lung tissue of a common vole (Microtus arvalis) with Tula hantavirus infection. Seven segments (L1–L3, M2–M3, S2, and S4) of the Hungarian MRV isolate MORV/47Ma/06 revealed a high similarity with an MRV strain detected in bank vole (Myodes glareolus) in Germany. The M1 and S3 segment of the Hungarian isolate showed the closest relationship with the sequence of a Slovenian human and a French murine isolate, respectively. The highest nucleotide and amino acid identity values were above 90 and 95% in all of the comparisons to the reference sequences in GenBank, except for the S1 with a maximum of 69.6% nucleotide and 75.4% amino acid identity. As wild rodents are among the main sources of zoonotic infections, the reservoir role of these animals and zoonotic potential of rodent origin MRVs need to be further investigated.
This is a preview of subscription content, log in via an institution to check access.
References
N. Decaro, M. Campolo, C. Desario, D. Ricci, M. Camero, E. Lorusso, G. Elia, A. Lavazza, V. Martella, C. Buonavoglia, Vet. Microbiol. 109, 19–27 (2005)
D. Lelli, A. Moreno, A. Steyer, T. Nagliˇc, C. Chiapponi, A. Prosperi, F. Faccin, E. Sozzi, A. Lavazza, Viruses 7, 5844–5854 (2015)
A. Steyer, I. Gutiérrez-Aguire, M. Kolenc, S. Koren, D. Kutnjak, M. Pokorn, M. Poljšak Prijatelj, N. Racki, M. Ravnikar, M. Sagadin, A.F. Steyer, N. Toplak, J. Clin. Microbiol. 51, 3818–3825 (2013)
Y.W. Zhang, Y. Liu, H. Lian, F. Zhang, S.F. Zhang, R.L. Hu, Arch. Virol. 161, 495–498 (2016)
Z. Li, D. Liu, X. Ran, C. Liu, D. Guo, X. Hu, J. Tian, X. Zhang, Y. Shao, S. Liu, L. Qu, Infect. Genet. Evol. 43, 347–353 (2016)
P.J. Johansson, T. Sveger, K. Ahlfors, J. Ekstrand, L. Svensson, Scand. J. Infect. Dis. 128, 117–120 (1996)
Z. Li, Y. Shao, C. Liu, D. Liu, D. Guo, Z. Qiu, J. Tian, X. Zhang, S. Liu, L. Qu, Infect. Genet. Evol. 36, 55–61 (2015)
A.T. Narayanappa, H. Sooryanarain, J. Deventhiran, D. Cao, B.A. Venkatachalam, D. Kambiranda, T. LeRoith, C.L. Heffron, N. Lindstrom, K. Hall, P. Jobst, C. Sexton, X.J. Meng, S. Elankumaran, MBio (2015). doi:10.1128/mBio.00593-15
F. Jakab, G. Horváth, E. Ferenczi, J. Sebok, G. Szucs, Arch. Virol. 153, 2093–2096 (2008)
R. Kugler, R.E. Marschang, K. Ihász, G. Lengyel, F. Jakab, K. Bányai, S.L. Farkas, Virus Res. 215, 94–98 (2016)
C.K. Johnson, P.L. Hitchens, T.S. Evans, T. Goldstein, K. Thomas, A. Clements, D.O. Joly, N.D. Wolfe, P. Daszak, W.B. Karesh, J.K. Mazet, Sci. Rep. 5, 14830 (2015)
A.D. Luis, D.T. Hayman, T.J. O’Shea, P.M. Cryan, A.T. Gilbert, J.R. Pulliam, J.N. Mills, M.E. Timonin, C.K. Willis, A.A. Cunningham, A.R. Fooks, C.E. Rupprecht, J.L. Wood, C.T. Webb, Proc. Biol. Sci. 280, 20122753 (2013)
Acknowledgements
The research activity of F.J. was supported by the TÁMOP 4.2.4. A/2-11-1-2012 0001—National Excellence Program Elaborating and operating an inland student and researcher personal support system. The project was subsidized by the European Union and co-financed by the European Social Fund. Research activity of G.K. and F.J. was supported by the ÚNKP-16-3-III and ÚNKP-16-4-III—New Excellence Program of the Ministry of Human Capacities. K.B. was supported by the “Momentum program” of the Hungarian Academy of Sciences (HAS). E.F., S.M., and S.L.F. were supported by the János Bolyai Research Scholarship of HAS. Research activity of K.K. was supported by the Szentágothai Talent Program, financed by the Szentágothai Research Centre, University of Pécs. The present scientific contribution is dedicated to the 650th anniversary of the foundation of the University of Pécs, Hungary.
Authors contribution
F.J. and K.B. conceived the study. M.O., K.K. and G.H. collected samples and analyzed the data. R.K., S.L.F. and S.M. carried out all the sequencing. E.F. and G.K. carried out the laboratory experiments, isolation, and wrote the manuscript. All authors read and approved the final manuscript.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare no conflict of interest and confirm that this manuscript is not currently under consideration by any other journal.
Ethical approval
This article does not contain any studies with animals performed by any of the authors.
Additional information
Edited by William Dundon.
Electronic supplementary material
Below is the link to the electronic supplementary material.
11262_2016_1411_MOESM1_ESM.pptx
Unrooted phylogenetic trees of mammalian orthoreovirus L, M, and S amino acid sequences. Black dots label sequences of the Hungarian MRV strain MORV/47Ma/06. The phylogenetic calculations were carried out using the neighbor-joining method and applying the best fit models, with 1000 bootstrap replicates. Bootstrap values <60 are shown. The scale bar represents the substitutions per site and is proportional to the genetic distance for each phylogenetic tree. (PPTX 388 kb)
Rights and permissions
About this article
Cite this article
Fehér, E., Kemenesi, G., Oldal, M. et al. Isolation and complete genome characterization of novel reassortant orthoreovirus from common vole (Microtus arvalis). Virus Genes 53, 307–311 (2017). https://doi.org/10.1007/s11262-016-1411-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-016-1411-1