Abstract
The lytic cold-active bacteriophage VSW-3, belonging to the Podoviridae family and infecting the host Pseudomonas fluorescens SW-3, was isolated from the Napahai plateau wetland in China. With the development of sequencing technology, the study of Pseudomonas genomic diversity has increased; however, knowledge of cold-active phages infecting Pseudomonas is limited. The newly sequenced phage VSW-3 was classified based on virion morphology by transmission electron microscope. Sequence analysis revealed that the genome size was 40,556 bp with an overall GC content of 57.54 % and 46 open reading frames. The genome was organized into several modules containing genes for packaging, structural proteins, replication/transcription, and phage lysis. The sequence contained 45 potential promoters, 3 transcription terminators, and yet no tRNAs. This is the first report of cold-active Pseudomonas fluorescens bacteriophage genome sequencing.
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Acknowledgments
This study was funded by the National Natural Science Foundation of China (31160121 and 31360129) and Personnel Training Fund by the Kunming University of Science and Technology (KKZ3201526010).
Author contributions
CJZ, XLJ, and YLW designed the experiments. CJZ, ZYZ, JKL, and KHQ carried out the experiments. CJZ, XLJ, QZ, and LBL analyzed the data. CJZ, ZYZ, XLJ, and YLW prepared the manuscript. All authors read and approved the final manuscript.
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Edited by Detlev H. Kruger.
Chunjing Zhang and Zhongyao Zhang are Co-first authors.
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Zhang, C., Zhang, Z., Li, J. et al. Complete genome sequence of the lytic cold-active Pseudomonas fluorescens bacteriophage VSW-3 from Napahai plateau wetland. Virus Genes 53, 146–150 (2017). https://doi.org/10.1007/s11262-016-1403-1
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DOI: https://doi.org/10.1007/s11262-016-1403-1