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Host–pathogen interactome analysis of Chikungunya virus envelope proteins E1 and E2

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Abstract

The envelope proteins of Chikungunya virus (CHIKV) are known to play crucial roles in viral infection and spread. Although the role of envelope proteins in viral infection has been studied, the cellular interactors of these proteins are still elusive. In the present study, the ectodomains of CHIKV envelope proteins (E1 and E2) have been used for a high throughput yeast two-hybrid (Y2H) screening to identify the interacting host protein partners. Following a comparative analysis between the viral–host protein interaction data generated from Y2H and computational approach, five host proteins interacting with E1 and three host proteins interacting with E2 common to both datasets were identified. These associations were further verified independently by pull down and protein interaction ELISA. The identified interactions shed light on the possible cellular machinery that CHIKV might be employing during viral entry, trafficking, and evasion of immune system.

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Acknowledgments

This work was funded by All India Council for Technical Education, Govt. of India (Grant No. 8023/BOR/RID/ROS/69/2008-09).

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Authors and Affiliations

  1. Center for Emerging Diseases, Department of Biotechnology, Jaypee Institute of Information Technology, A-10, Sector-62, Noida, 201307, UP, India

    Namrata Dudha, Jyoti Rana, Sreejith Rajasekharan, Reema Gabrani & Sanjay Gupta

  2. Department of Microbiology, University of Delhi South Campus, Benito Juarez Marg, New Delhi, 110021, India

    Amita Gupta

  3. Department of Biochemistry, University of Delhi South Campus, Benito Juarez Marg, New Delhi, 110021, India

    Vijay Kumar Chaudhary

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  1. Namrata Dudha
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Correspondence to Sanjay Gupta.

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Dudha, N., Rana, J., Rajasekharan, S. et al. Host–pathogen interactome analysis of Chikungunya virus envelope proteins E1 and E2. Virus Genes 50, 200–209 (2015). https://doi.org/10.1007/s11262-014-1161-x

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