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Detection and differentiation of Japanese encephalitis virus genotype I and genotype III by reverse transcription loop-mediated isothermal amplification combined with restriction fragment length polymorphism

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Abstract

Japanese encephalitis (JE), which is a mosquito-borne arboviral infection, is the leading cause of viral encephalitis in Asian countries. The causative agent of JE is Japanese encephalitis virus (JEV), in which the predominant genotype has changed from genotype III (G III) to genotype I (G I). However, a method for the rapid differentiation between JEV G I and G III remains unavailable. This study aimed to establish a rapid JEV genotyping method using reverse transcription loop-mediated isothermal amplification (RT-LAMP). An Spe I site, which was located in the target sequence (C gene) of JEV G III strains but not in JEV G I strains, was selected as the RT-LAMP target. After testing 64 specimens, results showed that RT-LAMP can detect and differentiate JEV G I and G III specifically. Thus, a novel RT-LAMP system for the rapid detection and differentiation of JEV G I and G III was developed successfully.

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Acknowledgments

This work was supported by a grant from the Special Fund for Agro-Scientific Research in the Public Interest (No. 201203082).

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Authors and Affiliations

  1. Swine Research Center, Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Ya’an, 625014, China

    Liang Zhang, Sanjie Cao, Rui Wu, Shuquan Zhu, Hanyang Liu, Lei Yuan, Shuangyan Shi, Dan Zhang, Xiaobo Huang, Xintian Wen, Yiping Wen, Qigui Yan, Yong Huang & Xiaoping Ma

  2. Laboratory of Zoonosis, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China

    Liang Zhang, Sanjie Cao, Rui Wu, Shuquan Zhu, Hanyang Liu, Lei Yuan, Shuangyan Shi, Dan Zhang, Xiaobo Huang, Xintian Wen, Yiping Wen, Qigui Yan, Yong Huang & Xiaoping Ma

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  1. Liang Zhang
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  2. Sanjie Cao
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  3. Rui Wu
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Correspondence to Sanjie Cao.

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Zhang, L., Cao, S., Wu, R. et al. Detection and differentiation of Japanese encephalitis virus genotype I and genotype III by reverse transcription loop-mediated isothermal amplification combined with restriction fragment length polymorphism. Virus Genes 50, 231–237 (2015). https://doi.org/10.1007/s11262-014-1158-5

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  • DOI: https://doi.org/10.1007/s11262-014-1158-5

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