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Phylogenetic analysis of Tomato spotted wilt virus (TSWV) NSs protein demonstrates the isolated emergence of resistance-breaking strains in pepper

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Abstract

Resurgence of Tomato spotted wilt virus (TSWV) worldwide as well as in Hungary causing heavy economic losses directed the attention to the factors contributing to the outbreak of this serious epidemics. The introgression of Tsw resistance gene into various pepper cultivars seemed to solve TSWV control, but widely used resistant pepper cultivars bearing the same, unique resistance locus evoked the rapid emergence of resistance-breaking (RB) TSWV strains. In Hungary, the sporadic appearance of RB strains in pepper-producing region was first observed in 2010–2011, but in 2012 it was detected frequently. Previously, the non-structural protein (NSs) encoded by small RNA (S RNA) of TSWV was verified as the avirulence factor for Tsw resistance, therefore we analyzed the S RNA of the Hungarian RB and wild type (WT) isolates and compared to previously analyzed TSWV strains with RB properties from different geographical origins. Phylogenetic analysis demonstrated that the different RB strains had the closest relationship with the local WT isolates and there is no conserved mutation present in all the NSs genes of RB isolates from different geographical origins. According to these results, we concluded that the RB isolates evolved separately in geographic point of view, and also according to the RB mechanism.

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Authors and Affiliations

  1. Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Road 15, 1022, Budapest, Hungary

    Asztéria Almási, Zsófia Csömör, Katalin Nemes, Katalin Salánki & István Tóbiás

  2. Budakert Kft., Bartók B. Road 41, 1114, Budapest, Hungary

    Gábor Csilléry

  3. Department of Plant Pathology, Corvinus University of Budapest, Ménesi Road 44, 1118, Budapest, Hungary

    Zsófia Csömör & László Palkovics

Authors
  1. Asztéria Almási
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  2. Gábor Csilléry
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  3. Zsófia Csömör
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  4. Katalin Nemes
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  5. László Palkovics
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  6. Katalin Salánki
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  7. István Tóbiás
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Correspondence to Katalin Salánki.

Electronic supplementary material

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11262_2014_1131_MOESM1_ESM.pdf

Online Resource 1 GenBank accession numbers and main information/characteristics of the TSWV strains implied in the molecular and phylogenetic analysis in this study. * RB: resistance-breaking; WT: wild type (PDF 256 kb)

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Almási, A., Csilléry, G., Csömör, Z. et al. Phylogenetic analysis of Tomato spotted wilt virus (TSWV) NSs protein demonstrates the isolated emergence of resistance-breaking strains in pepper. Virus Genes 50, 71–78 (2015). https://doi.org/10.1007/s11262-014-1131-3

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  • DOI: https://doi.org/10.1007/s11262-014-1131-3

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