Skip to main content
SpringerLink
Log in

Genomic characterization of a novel partitivirus infecting Aspergillus ochraceus

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Three double-stranded RNA (dsRNA) segments from Aspergillus ochraceus isolate FA 0611, designated as AoR1, AoR2, and AoR3, were cloned and sequenced. AoR1 was identical with AoV dsRNA 1 previously reported from A. ochraceus ATCC 28706, which putatively encoded an RNA-dependent RNA polymerase of an unidentified mycovirus. AoR2 was found to encode a putative viral capsid protein (CP) with 63% similarity to that of Penicillium stoloniferum virus S, which was detected from P. stoloniferum ATCC 14586. The function of AoR3 was unknown. The three segments were found to contain a conserved sequence at their 5′ termini, while an identical sequence was only found at the 3′ termini of AoR1 and AoR2. It is suggested that AoR1, AoR2, and AoR3 originate from an independent partitivirus infecting A. ochraceus. The novel virus is suggested to be Aspergillus ochraceus virus 1, AoV1.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. S.A. Ghabrial, K.W. Buck, B.I. Hillman, R.G. Milne et al., Partitiviridae, in Eighth report of the international committee on taxonomy of viruses, ed. by C.M. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, A.L. Ball (Elsevier/Academic Press, London, 2005)

    Google Scholar 

  2. J.W. Kim, E.Y. Choi, J.I. Lee, Virus Genes 31, 175–183 (2005). doi:10.1007/s11262-005-1793-y

    Article  PubMed  CAS  Google Scholar 

  3. R. Rong, S. Rao, S.W. Scott, G.R. Carner, F.H. Tainter, Virus Res. 90, 217–224 (2002). doi:10.1016/S0168-1702(02)00178-8

    Article  PubMed  CAS  Google Scholar 

  4. I.E. Tzanetakis, R. Price, R.R. Martin, Virus Genes 36, 267–272 (2008). doi:10.1007/s11262-007-0186-9

    Article  PubMed  CAS  Google Scholar 

  5. J.W. Kim, R.F. Bozarth, J. Gen. Virol. 66, 1991–2002 (1985). doi:10.1099/0022-1317-66-9-1991

    Article  CAS  Google Scholar 

  6. J.W. Kim, S.Y. Kim, K.M. Kim, Virus Genes 27, 249–256 (2003). doi:10.1023/A:1026343831909

    Article  PubMed  CAS  Google Scholar 

  7. J.W. Kim, E.Y. Choi, Y.T. Kim, Virus Res. 120, 212–215 (2006). doi:10.1016/j.virusres.2006.04.004

    Article  PubMed  CAS  Google Scholar 

  8. J. Varga, E. Rinyu, E. Kevei, B. Toth, Z. Kozakiewicz, Can. J. Microbiol. 44, 569–574 (1998). doi:10.1139/cjm-44-6-569

    Article  PubMed  CAS  Google Scholar 

  9. A.D. van Diepeningen, A.J. Debets, R.F. Hoekstra, Fungal Genet. Biol. 43, 446–452 (2006). doi:10.1016/j.fgb.2006.01.014

    Article  PubMed  Google Scholar 

  10. L. Chen, J.S. Chen, L. Liu, X. Yu, S. Yu, T.Z. Fu et al., Arch. Virol. 151, 849–859 (2006). doi:10.1007/s00705-005-0685-8

    Article  PubMed  CAS  Google Scholar 

  11. P.R. Lambden, S.J. Cooke, E.O. Caul, I.N. Clarke, J. Virol. 66, 1817–1822 (1992)

    PubMed  CAS  PubMed Central  Google Scholar 

  12. K.B. Nicholas, H.B. Nicholas, GeneDoc: a tool for editing and annotating multiple sequence alignments. Distributed by the authors (1997)

  13. S.F. Altschul, T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller et al., Nucleic Acids Res. 25, 3389–3402 (1997). doi:10.1093/nar/25.17.3389

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  14. J. Sambrook, D.W. Russell, Molecular Cloning: A Laboratory Manual, 3rd edn. (CSHL Press, New York, 2001)

    Google Scholar 

  15. C.V. Hacker, C.M. Brasier, K.W. Buck, Virus Genes 32, 137–138 (2006). doi:10.1007/s11262-005-6868-2

    Article  PubMed  CAS  Google Scholar 

  16. T.T. Tuomivirta, J. Hantula, Arch. Virol. 148, 2293–2305 (2003). doi:10.1007/s00705-003-0194-6

    Article  PubMed  CAS  Google Scholar 

  17. T.T. Tuomivirta, J. Hantula, Virus Res. 110, 31–39 (2005). doi:10.1016/j.virusres.2004.12.005

    Article  PubMed  CAS  Google Scholar 

  18. G. Boccardo, T. Candresse, Arch. Virol. 150, 399–402 (2005). doi:10.1007/s00705-004-0422-8

    Article  PubMed  CAS  Google Scholar 

  19. S.A. Ghabrial, Virus Genes 16, 119–131 (1998). doi:10.1023/A:1007966229595

    Article  PubMed  CAS  Google Scholar 

  20. N.M. Salem, D.A. Golino, B.W. Falk, A. Rowhani, Arch. Virol. 153, 455–462 (2008). doi:10.1007/s00705-007-0008-3

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (30671361). We thank Mr. Feng Xue for his assistance of the phylogenetic analysis and Dr. Xiufang Hu for identifying the fungus.

Author information

Author notes

  1. Weixia Liu

    Present address: Institute of Infectious Diseases, First Affiliated Hospital, Medical Faculty, Zhejiang University, No. 79 Qingchun Road, Hangzhou, Zhejiang, 310003, People’s Republic of China

Authors and Affiliations

  1. Institute of Bioengineering, Zhejiang Sci-Tech University, Road No. 2, Xiasha, Hangzhou, Zhejiang, 310018, People’s Republic of China

    Weixia Liu, Greg Duns & Jishuang Chen

Authors
  1. Weixia Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Greg Duns
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jishuang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jishuang Chen.

Additional information

The GenBank Accession numbers of the sequences reported in this article are EU118277, EU118278, and EU118279.

Electronic supplementary material

Below is the link to the electronic supplementary material.

MOESM1 (DOC 292 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, W., Duns, G. & Chen, J. Genomic characterization of a novel partitivirus infecting Aspergillus ochraceus . Virus Genes 37, 322–327 (2008). https://doi.org/10.1007/s11262-008-0265-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-008-0265-6

Keywords

Search

Navigation