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Characterization of VP1, VP2 and VP3 Gene Segments of A Human Rotavirus Closely Related to Porcine Strains

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Abstract

Long RNA electropherotype rotavirus strains with subgroup I specificity predominated the infantile gastroenteritis outbreak in Manipur, India, in 1987–88. One such strain (RMC321) was found to possess porcine characteristics in 7 out of 8 genes sequenced. Partial characterization of its remaining VP1, VP2 and VP3 genes along with a porcine rotavirus strain (HP140) uncovered their close genetic relation to porcine strains. VP7 was the only gene segment of this strain with significant genetic identity to human strains. This indicates that a rotavirus reassortant strain with most of its genetic material derived from a porcine strain may cause symptomatic infection in a human host.

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References

  1. A.Z. Kapikian, Y. Hoshino, R.M. Chanock, in Field’s virology, 4th edn, ed. by D.M. Knipe, P.M. Howley, D.E. Griffin, R.A. Lamb, M.A. Martin, B. Roizman, S.E. Straus (Lippincott. Williams and Wilkins, Philadelphia, 2001) pp 1787–1833

  2. R.F. Ramig (1997) Ann. Rev. Microbiol. 51 225–255 Occurrence Handle1:CAS:528:DyaK2sXms1Shsbk%3D Occurrence Handle10.1146/annurev.micro.51.1.225

    Article  CAS  Google Scholar 

  3. N. Kobayashi, M.M. Alam, K. Kojima, K. Mise, M. Ishino, A. Sumi, in Genomic diversity and molecular epidemiology of rotaviruses, ed. by N. Kobayashi (Research Signpost, Trivandrum, 2003) pp 39–54

  4. M.K. Estes, in Field’s virology, 4th edn, ed. by D.M. Knipe, P.M. Howley, D.E. Griffin, R.A. Lamb, B. Roizman, S.E. Straus (Lippincott. Williams and Wilkins, Philadelphia, 2001) pp 1747–1785

  5. S.K. Ghosh T.N Naik (1989) Arch. Virol. 105 119–127 Occurrence Handle2541674 Occurrence Handle1:STN:280:BiaB2cngt1I%3D Occurrence Handle10.1007/BF01311122

    Article  PubMed  CAS  Google Scholar 

  6. V. Varghese S. Das N.B. Singh K. Kojima S.K. Bhattacharya T. Krishnan N. Kobayashi T.N. Naik (2004) Arch. Virol. 149 155–172 Occurrence Handle14689281 Occurrence Handle1:CAS:528:DC%2BD3sXpvVKqtrs%3D Occurrence Handle10.1007/s00705-003-0199-1

    Article  PubMed  CAS  Google Scholar 

  7. C.Q. Zeng M.K. Estes A. Charpilienne J.J. Cohen (1998) Virology 72 201–208 Occurrence Handle1:CAS:528:DyaK1cXhvVCnsQ%3D%3D

    CAS  Google Scholar 

  8. A.M. Gonzalez T.V. Nguyen M.S. Azevedo K. Jeong F. Agarib C. Iosef K. Chang K. Lovgren-Bengtsson B. Morein L.J. Saif (2004) Clin. Exp. Immunol. 135 361–372 Occurrence Handle15008967 Occurrence Handle1:STN:280:DC%2BD2c7hvFSmug%3D%3D Occurrence Handle10.1111/j.1365-2249.2004.02395.x

    Article  PubMed  CAS  Google Scholar 

  9. M. Liu N.M. Mattion M.K. Estes (1992) Virology 188 77–84 Occurrence Handle1314468 Occurrence Handle1:CAS:528:DyaK3sXks1Omt7c%3D Occurrence Handle10.1016/0042-6822(92)90736-9

    Article  PubMed  CAS  Google Scholar 

  10. D. Chen C.L. Luongo M.L. Nibert J.T. Patton (1999) Virology 265 120–130 Occurrence Handle10603323 Occurrence Handle1:CAS:528:DyaK1MXotVSjsLc%3D Occurrence Handle10.1006/viro.1999.0029

    Article  PubMed  CAS  Google Scholar 

  11. S. Das V. Varghese S. Chaudhuri P. Barman K. Kojima P. Dutta S.K. Bhattacharya T. Krishnan N. Kobayashi T.N. Naik (2004) J. Med. Virol. 72 156–461 Occurrence Handle14635025 Occurrence Handle1:CAS:528:DC%2BD2cXht1Cgtg%3D%3D Occurrence Handle10.1002/jmv.10542

    Article  PubMed  CAS  Google Scholar 

  12. M. Labbe P. Baudoux A. Charpilienne D. Poncet J. Cohen (1994) J. Gen. Virol. 75 3423–3430 Occurrence Handle7996135 Occurrence Handle1:CAS:528:DyaK2MXisFKgsr8%3D Occurrence Handle10.1099/0022-1317-75-12-3423

    Article  PubMed  CAS  Google Scholar 

  13. J.P. Cook M.A. McCrae (2004) J. Gen. Virol. 85 929–932 Occurrence Handle15039535 Occurrence Handle1:CAS:528:DC%2BD2cXivF2mtbk%3D Occurrence Handle10.1099/vir.0.19629-0

    Article  PubMed  CAS  Google Scholar 

  14. P.J. Masendycz E.A. Palombo (2001) FEMS Microbiol. Lett. 198 147–150 Occurrence Handle11430406 Occurrence Handle1:CAS:528:DC%2BD3MXjtFGit74%3D

    PubMed  CAS  Google Scholar 

  15. C.L. Zao W.N. Yu C.L. Kao K. Taniguchi C.Y. Lee C.N. Lee (1999) J. Gen. Virol. 80 1407–1415 Occurrence Handle10374958 Occurrence Handle1:CAS:528:DyaK1MXjs1Omtbw%3D

    PubMed  CAS  Google Scholar 

  16. O. Nakagomi T. Nakagomi (1996) Arch. Virol. [Suppl] 12 93–98 Occurrence Handle1:STN:280:ByiC28jktFM%3D

    CAS  Google Scholar 

  17. Y. Qian K.Y. Green (1991) Virology 182 407–412 Occurrence Handle1850912 Occurrence Handle1:CAS:528:DyaK3MXmslGgs7g%3D Occurrence Handle10.1016/0042-6822(91)90691-4

    Article  PubMed  CAS  Google Scholar 

  18. N.A. Cunliffe P.A. Woods J.P. Leite B.K. Das M. Ramachandran M.K. Bhan C.A. Hart R.I. Glass J.R. Gentsch (1997) J. Med. Virol. 53 41–50 Occurrence Handle9298731 Occurrence Handle1:CAS:528:DyaK2sXmtVGjsLw%3D Occurrence Handle10.1002/(SICI)1096-9071(199709)53:1<41::AID-JMV8>3.0.CO;2-Q

    Article  PubMed  CAS  Google Scholar 

  19. J.R. Gentsch B.K. Das B. Jiang M.K. Bhan R.I. Glass (1993) Virology 194 424–430 Occurrence Handle8386888 Occurrence Handle1:CAS:528:DyaK3sXks1Onu7s%3D Occurrence Handle10.1006/viro.1993.1280

    Article  PubMed  CAS  Google Scholar 

  20. E.A. Palombo (2002) Virus Genes 24 11–20 Occurrence Handle11928984 Occurrence Handle1:CAS:528:DC%2BD38XisVShtLs%3D Occurrence Handle10.1023/A:1014073618253

    Article  PubMed  CAS  Google Scholar 

  21. S.J. Dunn T.L. Cross H.B. Greenberg (1994) Virology 203 178–183 Occurrence Handle8030275 Occurrence Handle1:CAS:528:DyaK2MXjt1enuw%3D%3D Occurrence Handle10.1006/viro.1994.1471

    Article  PubMed  CAS  Google Scholar 

  22. C.D. Rao M. Dos P. Ilango R. Lalwani B.S. Rao K. Gowda (1995) Virology 207 327–333 Occurrence Handle7871749 Occurrence Handle1:CAS:528:DyaK2MXjslCmtbw%3D Occurrence Handle10.1006/viro.1995.1087

    Article  PubMed  CAS  Google Scholar 

  23. V. Gouvea L. Castro Particlede C. Timenetsky Mdo H. Greenberg N. Santos (1994) J. Clin. Microbiol. 32 1408–1409 Occurrence Handle8051281 Occurrence Handle1:STN:280:ByuA38jnvFc%3D

    PubMed  CAS  Google Scholar 

  24. J.P. Leite A.A. Alfieri P.A. Woods R.I. Glass J.R. Gentsch (1996) Arch. Virol. 141 2365–2374 Occurrence Handle9526543 Occurrence Handle1:CAS:528:DyaK2sXpt1Kgtg%3D%3D Occurrence Handle10.1007/BF01718637

    Article  PubMed  CAS  Google Scholar 

  25. C.D. Kirkwood E.A. Palombo (1997) Virology 236 258–265 Occurrence Handle9325233 Occurrence Handle1:CAS:528:DyaK2sXmsFeqsL4%3D Occurrence Handle10.1006/viro.1997.8727

    Article  PubMed  CAS  Google Scholar 

  26. R.B. Bellinzoni N.M. Mattion D.O. Matson J. Blackhall J.L. La Torre E.A. Scodeller S. Urasawa K. Taniguchi M.K. Estes (1990) J. Clin. Microbiol. 28 633–636 Occurrence Handle2157739 Occurrence Handle1:STN:280:By%2BB3MjgvVA%3D

    PubMed  CAS  Google Scholar 

  27. N. Santos R.C. Lima C.M. Nozawa R.E. Linhares V. Gouvea (1999) J. Clin. Microbiol. 37 2734–2736 Occurrence Handle10405435 Occurrence Handle1:CAS:528:DyaK1MXltVSqsrY%3D

    PubMed  CAS  Google Scholar 

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Acknowledgements

The research was funded by Indian Council of Medical Research (ICMR), New Delhi, India. V. Varghese, S. Ghosh and S. Das were supported by Senior Research Fellowships from Indian Council of Medical Research and Council of Scientific and Industrial Research, New Delhi, India.

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Authors and Affiliations

  1. Division of Virology, National Institute of Cholera and Enteric Diseases, C.I.T. Road, Scheme XM, Beliaghata, P-33, 700010, Kolkata, India

    Vici Varghese, Souvik Ghosh, Soma Das, Triveni Krishnan & Trailokya Nath Naik

  2. Division of Clinical Medicine, National Institute of Cholera and Enteric Diseases, 700010, Kolkata, India

    Sujit Kumar Bhattacharya

  3. Department of Life Science and Biotechnology, Jadavpur University, Jadavpur, 700032, Kolkata, India

    Parimal Karmakar

  4. Department of Hygiene, Sapporo Medical University School of Medicine, 060, Sapporo, Japan

    Nobumichi Kobayashi

  5. ICMR Virus Unit, GB4, ID and BG Hospital Campus, 700010, Kolkata, India

    Sujit Kumar Bhattacharya & Trailokya Nath Naik

Authors
  1. Vici Varghese
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  2. Souvik Ghosh
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  3. Soma Das
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  4. Sujit Kumar Bhattacharya
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  5. Triveni Krishnan
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  6. Parimal Karmakar
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  7. Nobumichi Kobayashi
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  8. Trailokya Nath Naik
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Corresponding author

Correspondence to Trailokya Nath Naik.

Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned accession numbers AY601114 and AY601117 for VP1, AY601115 and AY601118 for VP2 gene and AY601116 and AY601119 for VP3 gene of RMC321 and HP140 respectively.

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Varghese, V., Ghosh, S., Das, S. et al. Characterization of VP1, VP2 and VP3 Gene Segments of A Human Rotavirus Closely Related to Porcine Strains. Virus Genes 32, 241–247 (2006). https://doi.org/10.1007/s11262-005-6908-y

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  • DOI: https://doi.org/10.1007/s11262-005-6908-y

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