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Murine Gammaherpesvirus (MHV) MK3 Gene Sequence Diversity among 72, 4556, and 68 Strains

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Abstract

Murid herpesvirus 4 (MuHV-4) currently serves as a model for study of human gamma-herpesvirus pathogenesis. It codes for MK3 protein that similarly as K5 protein of Kaposi’s sarcoma-associated herpesvirus are members of a family of structurally related viral immune evasion molecules possessing RING-CH finger domain with ubiquitin ligase activity. Murine herpesvirus 72 (MHV-72) isolated from the same species of free-living small rodent is considered as closely related to Murine herpesvirus 68 (MHV-68). Studies on MHV-72, identified dissimilarity from MHV-68 in the sequence of glycoprotein 150 [K. Mačáková, J. Matis, I. Režuchová, O. Kúdela, H. Rašlová, M. Kúdelová, Virus Genes 26, 89–95 (2003)]. Murine herpesvirus 4556 (MHV-4556) is relatively new, till now, uncharacterised strain isolated from different murid species Apodemus flavicollis. We have therefore sequenced the MK3 gene of MHW-72 as well as of MHV-4556 to find out the evidence of their difference from that of MHV-68. We show here the unique nucleotide mutation in MHV-72 MK3 gene changing the codon at C-end of MK3 protein that was earlier predicted to function in interaction with TAP1/2. Furthermore, one from two nucleotide mutations found for MHV-4556 MK3 gene changed the codon that is localized at N-terminus of MK3 protein. MHV-4556-specific mutation was found within MK3 RING-CH finger domain known to be necessary for the ubiquitination of MHC class I proteins. Moreover, the latter established the new restriction site specific for MHV-4556.

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Acknowledgement

This work was supported by VEGA, the joint grant agency of Slovak Ministry of Education and Slovak Academy of Sciences (#2/4121/04) and by National program of Research and Development (#SP 51/028 08 00/028 08 03) and ATVV (#51 – 005 005).

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Authors and Affiliations

  1. Institute of Virology, Slovak Academy of Sciences, 845 05, Bratislava, Slovak Republic

    Moniká Valovičová, Ingeborg Režuchorá, Katarína Mačáková, Petra Petrová, Ján Matis & Marcela Kúdelová

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  1. Moniká Valovičová
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  2. Ingeborg Režuchorá
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  3. Katarína Mačáková
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  4. Petra Petrová
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  5. Ján Matis
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  6. Marcela Kúdelová
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Corresponding author

Correspondence to Marcela Kúdelová.

Additional information

The nucleotide sequence data of the MK3 gene of MHV-72 and of MHV-4556 sequence have been submitted to the GenBank under Accession Numbers DQ124247 and DQ 124248.

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Valovičová, M., Režuchorá, I., Mačáková, K. et al. Murine Gammaherpesvirus (MHV) MK3 Gene Sequence Diversity among 72, 4556, and 68 Strains. Virus Genes 33, 51–58 (2006). https://doi.org/10.1007/s11262-005-0038-4

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