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Effect of Edwardsiella tarda immunization on systemic immune response, mucosal immune response and protection in catla (Catla catla)

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Abstract

The effect of immunization on systemic and cutaneous mucosal immune responses of fish and their possible relation with protection has not been fully assessed. In this study, healthy catla (Catla catla) were immunized against Edwardsiella tarda using two antigenic preparations namely, whole cell bacterin (B) and bacterin mixed with Freund’s complete adjuvant in a 1:1 (v/v) ratio (B+A) followed by a booster dose after 3 weeks of first injection. Different systemic and cutaneous mucosal immune responses were measured at weekly interval upto 8th week post vaccination (pv). Fish were challenged 8 weeks pv with live E. tarda to study vaccine induced protection. The result showed that although there were strong systemic as well as mucosal immune responses, particularly after booster dose, the challenge produced low to moderate protection in terms of relative percent survival (RPS). The maximum RPS (50 %) was recorded in the adjuvanted bacterin group after 8 weeks pv. Low to moderate protection after challenge, which may be attributed to the intracellular nature of E. tarda and/or use of crude antigenic preparation, accounts for new strategy to be developed for immunization programme against such intracellular pathogen. The results collectively suggest possible involvement of systemic as well as mucosal immune responses in inducing protective immunity in catla.

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Acknowledgments

The authors are thankful to Vice Chancellor, Central Agricultural University, Imphal, India and Dean, College of Fisheries, CAU, Lembucherra, Tripura, India for providing necessary research facilities.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Fish Health and Environment, College of Fisheries, Central Agricultural University, Lembucherra, Tripura (W), 799 210, Tripura, India

    Khriezhato Nakhro, Anushree Das & Dibyendu Kamilya

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  1. Khriezhato Nakhro
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  2. Anushree Das
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  3. Dibyendu Kamilya
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Correspondence to Dibyendu Kamilya.

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Nakhro, K., Das, A. & Kamilya, D. Effect of Edwardsiella tarda immunization on systemic immune response, mucosal immune response and protection in catla (Catla catla). Vet Res Commun 38, 115–122 (2014). https://doi.org/10.1007/s11259-014-9593-2

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