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Protective impact of resveratrol in experimental rat model of hyperoxaluria

  • Urology - Original Paper
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Abstract

Purpose

Resveratrol (RES) is a polyphenol with antioxidant, anti-inflammatory, and many other physiological effects on tissues. In the present study, the effect of resveratrol in hyperoxaluria driven nephrolithiasis/nephrocalcinosis is investigated.

Methods

Wistar-Albino rats of 250–300 g (male, n = 24) were included in the present study. The rats were randomized into three groups: Group 1 consisted of the controls (n = 8), Group 2 of hyperoxaluria (1% ethylene glycol (EG), n = 8), and Group 3 of the treatment (1% EG + 10 mg/kg of RES, n = 8) group. At the beginning and fifth week of the study, two rats from each group were placed in metabolic cages for 24 h and their urine was collected. At the end of the study, the rats were killed and their blood was collected from the vena cava inferior. The right kidneys of the rats were used for biochemical and the left ones for immunohistochemical analyzes. Malondialdehyde (MDA), catalase, urea, calcium, oxalate, and creatinine clearance were studied in the blood, urine, and kidney tissues. Moreover, routine histological evaluation, and p38-MAPK and NFkB immunohistochemical analyses were conducted.

Results

In the hyperoxaluria group, urinary oxalate levels were higher than the control group; yet, lower in the treatment group compared to hyperoxaluria group (p < 0.05). Serum MDA levels in the hyperoxaluria group were higher than the control group; but in the treatment group it is lower than the hyperoxaluria group (p < 0.05). P38 MAPK activity was higher in the hyperoxaluria group compared to the control (p < 0.05). However, in terms of p38 MAPK activity, there were no statistically significant difference between hyperoxaluria and the treatment group (p < 0.069). Whereas NFkB activity in the hyperoxaluria group is higher than the control (p < 0.001), no statistically significant difference was observed with the treatment group.

Conclusions

In the present study, resveratrol was seen to prevent hyperoxaluria. With preventing oxidative stress factors and Randall plaque formation caused by free oxygen radicals, resveratrol can be an alternative treatment option that can increase the success rate in preventing stone recurrence in the future.

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Abbreviations

EG:

Ethylene glycol

RES:

Resveratrol

MDA:

Malondialdehyde

MAPK:

Mitogen activated protein kinase

NFkB:

Nuclear factor kappa B

CaOx:

Calcium oxalate

SOD:

Superoxide dismutase

ROS:

Reactive oxygen species

TBARS:

Thiobarbituric acid reactive substance

JNK:

C-Jun N-terminal kinase

AP-1:

Active protein-1

OPN:

Osteopontin

XO:

Xanthine oxidase

TNF:

Tumor necrosis factor

PKC:

Protein kinase C

SPSS 11.0:

Statistical Package for Social Sciences 11.0

CAT:

Catalase

ESWL:

Extra shock wave lithotripsy

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Author information

Authors and Affiliations

  1. Department of Urology, Medical Faculty, Süleyman Demirel University, Isparta, Turkey

    Taylan Oksay & Alper Özorak

  2. Department of Urology, Afyonkarahisar State Hospital, Afyonkarahisar, Turkey

    Sedat Yunusoğlu

  3. Department of Biochemistry, Medical Faculty, Suleyman Demirel University, Isparta, Turkey

    Mustafa Calapoğlu

  4. Department of Histology and Embryology, Medical Faculty, Süleyman Demirel University, Isparta, Turkey

    İ. Aydın Candan

  5. Department of Medical Biology, Medical Faculty, Süleyman Demirel Uviversity, Isparta, Turkey

    İbrahim Onaran

  6. Department of Urology, Urology Policlinic, Konya Training and Research Hospital, PK 42090, Hacı Şaban Mah. Meram Yeniyol Caddesi No: 97, Meram, Konya, Turkey

    Osman Ergün

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  1. Taylan Oksay
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Correspondence to Osman Ergün.

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The authors declare that they have no conflict of interests.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. The study design was approved by the Süleyman Demirel University Animal Experiments Local Ethic Board, Turkey.

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Oksay, T., Yunusoğlu, S., Calapoğlu, M. et al. Protective impact of resveratrol in experimental rat model of hyperoxaluria. Int Urol Nephrol 49, 769–775 (2017). https://doi.org/10.1007/s11255-017-1534-x

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  • DOI: https://doi.org/10.1007/s11255-017-1534-x

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