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The effect of pentoxifylline on detrusor muscle contractility after partial urethral obstruction in a rat model

  • Urology - Original Paper
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Abstract

Purpose

To evaluate the effect of pentoxifylline (PTX), a nonspecific type 4 phosphodiesterase inhibitor, on contractility of obstructed detrusor muscles.

Methods

Sixty male Sprague–Dawley rats were randomized into three groups each containing 20 animals. The control groups included rats with induced BOO which received the solvent of PTX (drinking water), the sham group included rats with intact bladder outlet which received no treatment, and the treatment group was treated with PTX after induction of BOO. Four weeks after the operation and/or treatment, animals were killed and detrusor muscle of them was evaluated for contractility parameters.

Results

Carbachol-induced detrusor muscle tension increased proportionally with concentration of carbachol. The maximum carbachol-inducing muscle tension was significantly higher in the sham group in comparison to the rats with BOO (P < 0.001). In the latter group, PTX treatment caused no significant improvement in the maximum carbachol-induced muscle tension compared to the control group. However, no significant difference was observed among the study groups concerning the pEC50 of carbachol (5.77 ± 0.10, 5.96 ± 0.64, and 5.84 ± 0.17 in the sham, control, and treatment groups, respectively). BOO resulted in a great but statistically non-significant (P = 0.074) decrease in electrically induced bladder contraction in comparison with the sham group. Treatment with PTX reversed this effect significantly (P = 0.023), and the obtained values were almost the same as those of the sham group.

Conclusion

PTX improved detrusor muscle contractions in responses to electric stimulation in obstructed bladder. In contrast, no improvement was detected in contractile responses to the carbachol stimulation.

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Acknowledgments

The present article was extracted from the thesis written by Soheil Moghtadernejad financially supported by Shiraz University of Medical Sciences. Hereby, the authors would like to thank Ms. Azam Bagheri for her technical assistance. They are also grateful for Ms. A. Keivanshekouh at the Research Improvement Center of Shiraz University of Medical Sciences for improving the use of English in the manuscript.

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Authors and Affiliations

  1. Department of Urology, Faghihi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

    Mehdi Shirazi, Soheil Moghtadernejad & Alireza Aminsharifi

  2. Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

    Hossein Mirkhani

  3. Department of Pharmacology, Shiraz University of Medical Sciences, Zand Street, P.O. Box 71344, Shiraz, Iran

    Hossein Mirkhani

  4. Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran

    Ahmad Monabbati

Authors
  1. Mehdi Shirazi
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  2. Hossein Mirkhani
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  4. Soheil Moghtadernejad
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Correspondence to Hossein Mirkhani.

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Shirazi, M., Mirkhani, H., Monabbati, A. et al. The effect of pentoxifylline on detrusor muscle contractility after partial urethral obstruction in a rat model. Int Urol Nephrol 47, 1493–1497 (2015). https://doi.org/10.1007/s11255-015-1049-2

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  • DOI: https://doi.org/10.1007/s11255-015-1049-2

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