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Dietary effects of commercial probiotics on growth performance, digestibility, and intestinal morphometry of broiler chickens

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Abstract

This study compared five commercially available probiotics vis-à-vis antibiotic growth promotant (AGP) supplementation and absence of feed additive based on efficiency, intestinal morphometry, and energy digestibility in improving broiler chicken production. A total of 630 straight run (Cobb) day-old broiler chicks were distributed to seven treatments following a completely randomized design, with ten replicates per treatment and nine birds per replicate per cage. Dietary treatments consisted of basal diet in combination with the following: without probiotics and AGP supplementation (treatment 1); 75 ppm each of chlorotetracycline (CTC) and Zn bacitracin (treatment 2); probiotic A, Bacillus subtilis (treatment 3); probiotic B, Bacillus subtilis (treatment 4); probiotic C, Enterococcus faecium (treatment 5); and probiotic D, Bacillus subtilis (treatment 6); probiotic E, Enterococcus faecium, Bifidobacterium spp., Pediococcus spp., and Lactobacillus spp. (treatment 7). At day 42, energy digestibility was determined by fasting three randomly selected birds from each treatment for 12 h and then subjecting them to their corresponding dietary treatments. Excreta were collected and pooled after 24 h of feeding. Pooled excreta were weighed, oven-dried, and subjected to energy analyses after 3-day collection. Apparent total tract metabolizable energy was then computed. At day 47, three birds were randomly selected per treatment for intestinal morphometry (villi height and crypt depth) of the duodenum, jejunum, and ileum. Dietary supplementation using probiotics showed no significant effect on overall body weight, weight gain, feed consumption, feed efficiency, dressing percentage, mortality, harvest recovery, carcass quality parameters (e.g., meat to bone ratio and abdominal fat content), intestinal morphometry, and energy digestibility. Birds under treatment 7 (basal feed + probiotic E) generated the highest income over feed and chick cost.

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References

  • Acar, J.F. and Mouglin G. 2006. Antimicrobial resistance at farm level. Revve Scientifque et Technique, 25(2), 775–792.

    Article  CAS  Google Scholar 

  • Aceret, A.G. 1988. Commercial growth promotants on the performance of broilers (MS Thesis, University of the Philippines Los Baños).

  • Awad W.A., Ghareeb K, Abdel-Raheem S, Böhm J. 2009. Effects of Dietary Inclusion of Probiotic and Synbiotic on Growth Performance, Organ Weights, and Intestinal Histomorphology of Broiler Chickens. Poultry Science; 88(1):49–56. https://doi.org/10.3382/ps.2008-00244.

    Article  CAS  PubMed  Google Scholar 

  • Bai Sp, Wu Am, Ding Xm, Lei Y, Bai J, Zhang Ky, Chio Js. 2013. Effects of probiotic-supplemented diets on growth performance and intestinal immune characteristics of broiler chickens. Poultry Science, 92(3), 663–70. https://doi.org/10.3382/ps.2012-02813.

    Article  CAS  PubMed  Google Scholar 

  • Balotoc, A.T. 1992. Zinc bacitracin, zeolite and live yeast culture in broiler diets. (Unpublished Undergraduate Thesis, University of the Philippines Los Baños).

  • Cavazzoni, V., Adami, A., and Castrovalt, C. 1998. Performance of broiler chickens supplemented with Bacillus coagulants as probiotics. British Poultry Science, 39, 526–529.

    Article  CAS  PubMed  Google Scholar 

  • Dizaji, B. R., Zakeri, A., Golbazfarsad, A., Faramarzy, S., and Ranjbari, O. 2013 Influences of Different Growth Promoters on Intestinal Morphology of Broiler Chickens. European Journal of Experimental Biology, 3(2):32–37

    CAS  Google Scholar 

  • Flores, H.R. 2003. Effects of different batches of liquid probiotics on the performance of broilers (Unpublished BS Thesis, University of the Philippines Los Baños).

  • Fuller, R. 2001. The chicken gut microflora and probiotic supplements. Journal of Poultry Science, 38, 189–196.

    Article  Google Scholar 

  • Jin, L.Z., Ho, Y.W., Abdula, N. Jalaludins S. 2000. Digestive and bacterial enzyme activities in broiler fed diets supplemented with Lactobacillus cultures. Poultry Sciences, 79, 886–891.

    Article  CAS  Google Scholar 

  • Jung, S.J., Houde, R., Baurhoo, B., Zhao, X., Le, B.H. 2008. Effects of galacto-oligosaccharides and a Bifidobacteria lactis-based probiotic strain on the growth performance and fecal microflora of broiler chickens. Poultry Sciences, 87, 1694–1699.

    Article  CAS  Google Scholar 

  • Lope, C. 2003. The performance of broilers fed with probiotics in drinking water, in feed or both. (Unpublished BS Thesis, University of the Philippines Los Baños).

  • Mills, S., Stanton, C., Fitzgerald, G.F. and Ross, R.P. 2011. Enhancing the stress responses of probiotics for a lifestyle from gut to product and back again. Microbial Cell Factories, 10(1), 1–15.

    Article  CAS  Google Scholar 

  • Mohan, B., Kadirvel, R., Natarahan, A., Bhaskaran, M. 1996. Effect of probiotic supplementation on growth, nitrogen utilization and serum cholesterol in broilers. British Poultry Science, 37, 395–401.

    Article  CAS  PubMed  Google Scholar 

  • Mohnl, M. 2006. Benefits from using biomin c-x and biomin imbo in poultry production. Biomin Newsletter, 4–37.

  • Mountzouris, K.C., P. Tsirtsikos, E. Kalamara, S. Nitsch, G. Schatmayr, and K. Fegeros. 2007. Evaluation of the Efficacy of a Probiotic Containing Lactobacillus, Bifido bacterium, Enterococcus, and Pediococcus strains in Promoting Broiler Performance and Modulating Cecal Microflora Composition and Metabolic Activities. Poultry Science 86:309–317

    Article  CAS  PubMed  Google Scholar 

  • Nunes R.V., C. Scherer, P. C. Pozza, Cinthia Eyng, L.D.G. Bruno, And F.M. Vietes. 2012. Use of Probiotics to Replace Antibiotics for Broilers. Revista Brasileira de Zootecnia, 41 (10), 2219–2224. https://doi.org/10.1590/S1516-359820120010000012

    Article  Google Scholar 

  • Olnood, G., Beski S.M.S, Choct, M., And Iji, P.A. 2015. Novel Probiotics: Their Effects on Growth Performance, Gut Development, Microbial Community and Activity of Broiler Chickens. Chinese Association of Animal Science and Veterinary Medicine Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co.,Ltd.

  • Otutumi, L.K., Gois, B.M., Garcia E.R. and Loddi, M.M. 2012. Variations on the efficacy of probiotics in poultry. Probiotic in Animals, Everlon Cid Rigobelo, IntechOpen. https://doi.org/10.5772/50058.

  • Panda, A.K., Reddy, M.R., Ramarao, S.V. and Praharaj, N.K. 2000. Effect of dietary supplementation of probiotic on performance and immune response of layers in decline phase of production. Indian Journal of Poultry Science, 3, 102–104.

    Google Scholar 

  • Pelicia, K., Mendes, A.A., Saldanha E.S.P.B., Pizzolante, C.C., Takashi, S.E., Moreira, J., Garcia, R.G., Quinteiro, R.R., Paz, I.C.L.A., and Komiyama, C.M. 2004. Use of prebiotics and probiotics of bacterial and yeast origin for free-range broiler chickens. Brazilian Journal of Poultry Science. https://doi.org/10.1590/S1516-635X2004000300006.

  • Piao X.S., Han, I.K., Kim, J.H., Cho, W.J., Kim, Y.H., Chao, L. 1999. Effects of enzyme, phytase, and yeast supplementation on the growth performance and pollution reduction of chickens. Applied and Environmental Microbiology Journal, 65(11), 4981–6.

    Google Scholar 

  • Ramlah, A.H. and Tan, C.K. 1995. Effects of probiotic supplementation on broiler performance. Perlanika Journal of Tropical Agricultural Science, 18(2), 109–112.

    Google Scholar 

  • Roughani, E., Arab, M. and Akbarian, A. 2007. Effects of probiotic and other feed additives on performance and immune response of broiler chicks. Department of Animal Science, Shiraz University, Shiraz, Iran.

    Google Scholar 

  • Salianeh, N., Shirzad, M.R., Seifi, S.J. 2011. Performance and antibody response of broiler chickens fed diets containing probiotic and prebiotic. Journal of Applied Animal Research, 39(1), 65–67.

    Article  CAS  Google Scholar 

  • Samli, H.E., Senkoylu, N., Koc, M., Kanter, M,. and Agma, A. 2007. Arch. Effect of a dietary supplementation combining a probiotic and a natural anticoccidial in broiler chickens. Journal of Animal Physiology and Animal Nutrition, 61(1), 42–49.

    Google Scholar 

  • Santoso, U., Tanaka, K. and Othani S. 1995. Effects of dried Bacillus cultures on growth of lipogenic enzymes in female chicks. British Journal, 7, 123–129.

    Google Scholar 

  • Singh, S. K, Niranjan, P. S, Singh, U. B, Koley S, Verma, D.N. 2009. Effects of dietary supplementation of probiotics on broiler chicken. Animal Nutrition and Feed Technology, 9, 23–24.

    Google Scholar 

  • Snel, J. H., J. M. Harmsen, P. W. Van De Wielen, And B. A. Williams. 2002. Dietary strategies to influence the gastrointestinal microflora of young animals, and its potential to improve intestinal health. Pages 37–69 in Nutrition and Health of the Gastrointestinal Tract. M. C. Block, ed. Wageningen Academic Publishing, Wageningen, the Netherlands.

    Google Scholar 

  • Talebi, A., Amirzadeh, B., Mokhtar, B., and Gahri, H. 2008. Production of organic acids by probiotic Lactobacilli can be used to reduce pathogen load in poultry. PLOS ONE, 37(5), 509–512.

    CAS  Google Scholar 

  • Tanaka, K. and Santoso, S. 2000. Fermented product from Bacillus subtillis inhibits lipid accumulation and ammonia production of broiler chickens. Asian-Australasian Journal of Animal Science, 13, 78–80.

    Google Scholar 

  • Weis, J., Hrnčár, C., Pál, G., Baraňska, B., Bujko, J., and Malíková, L. 2011. Effect of probiotic strain Enterococcus faecium M74 supplementation on the carcass parameters of different hybrid combination chickens. Scientific Papers: Animal Science and Biotechnologies, 44(1). https://www.researchgate.net/publication/266440078_Effect_of_Probiotic_Strain_Enterococcus_faecium_M74_Supplementation_on_the_Carcass_Parameters_of_Different_Hybrid_Combination_Chickens 

  • Zhang, Z.F. and I.H. Kim. 2014. Effects of Multistrain Probiotics on Growth Performance, Apparent Ileal Nutrient Digestibility, Blood Characteristcs, Cecal Microbial Shedding, and Excreta Odor Contents in Broilers. Poultry Science 93 :364–370

    Article  CAS  PubMed  Google Scholar 

  • Zulkifli, I.A., Mohd, N.N., Ho, Y.W. 2000. Growth performance and immune response of two commercial Lactobacillus cultures and oxytetracycline under heat stress condition. British Poultry Science, 41, 593–597.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. University of the Philippines Rural High School, College of Arts and Sciences, University of the Philippines Los Baños, Los Baños, Laguna, Philippines

    Precious Jewel D. dela Cruz

  2. Institute of Animal Science, College of Agriculture and Food Science, University of the Philippines Los Baños, Los Baños, Laguna, Philippines

    Clarita T. Dagaas, Amado A. Angeles & Oliver D. Abanto

  3. Los Baños, Laguna, Philippines

    Karen Mae M. Mangubat

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  1. Precious Jewel D. dela Cruz
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  2. Clarita T. Dagaas
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  3. Karen Mae M. Mangubat
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  4. Amado A. Angeles
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Correspondence to Precious Jewel D. dela Cruz.

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dela Cruz, P.J.D., Dagaas, C.T., Mangubat, K.M.M. et al. Dietary effects of commercial probiotics on growth performance, digestibility, and intestinal morphometry of broiler chickens. Trop Anim Health Prod 51, 1105–1115 (2019). https://doi.org/10.1007/s11250-018-01791-0

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