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Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil

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Abstract

Bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. Although the infection has been described in cattle in some countries, including in Brazil, there are only two reports of infection in buffaloes: one in Pakistan and one in Cambodia. The aim of the present study was to survey the occurrence of bovine immunodeficiency virus (BIV) in water buffaloes from the Amazon region, Pará state, Brazil. BIV proviral DNA was surveyed in 607 whole blood samples of water buffaloes from 10 farms located in the state of Pará using semi-nested polymerase chain reaction (PCR) (PCR-SN) to amplify the pol region of the viral genome. Of the 607 samples tested, 27 (4.4 %) were positive for BIV proviral DNA. The amplified fragments were confirmed by sequence analysis after cloning and nucleotide sequencing. The sequence obtained had 99 % similarity to the reference strain (R-29). The present study provides important epidemiological data because BIV was detected for the first time in water buffaloes in Brazil. Further, the results suggest the possibility of the virus being a risk factor for herd health because it may be a potential causal agent of chronic disease and, also may be associated to other infectious diseases.

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Acknowledgments

The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes), and INCT-Pecuaria for funding this work and the Pró Reitoria de Pesquisa da Universidade Federal do Pará (UFPA), the Programa de Pós Graduação em Ciência Animal da UFPA, the colleagues of the Clínica de Grandes Animais da UFPA, and the Laboratório de Retroviroses (RetroLab) da Universidade Federal de Minas Gerais (UFMG) for technical support.

Conflicts of interest

The authors declare no conflicts of interest.

Statement of animal rights

All procedures and animal handling followed the ethical principles in Animal Experimentation, adopted by the Ethics Committee in Animal Experimentation of UFMG/CEUA, under Protocol n° 133/2012.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Authors and Affiliations

  1. Instituto de Medicina Veterinária, Hospital Veterinário de Grandes Animais, Universidade Federal do Pará, Rodovia BR 316 Km 61, Bairro Saudade, 68740-970, Castanhal, PA, Brazil

    Tatiane Teles Albernaz, Rafaela das Mercês Silva & José Diomedes Barbosa

  2. Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil

    Rômulo Cerqueira Leite, Jenner Karlison Pimenta Reis, Ana Paula de Sousa Rodrigues, Telissa da Cunha Kassar, Claudia Fideles Resende & Cairo Henrique Sousa de Oliveira

  3. Instituto de Biodiversidade e Florestas, Universidade Federal do Oeste do Pará, Santarém, PA, Brazil

    Felipe Masiero Salvarani

Authors
  1. Tatiane Teles Albernaz
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  2. Rômulo Cerqueira Leite
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  3. Jenner Karlison Pimenta Reis
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  4. Ana Paula de Sousa Rodrigues
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  5. Telissa da Cunha Kassar
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  6. Claudia Fideles Resende
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  7. Cairo Henrique Sousa de Oliveira
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  8. Rafaela das Mercês Silva
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  9. Felipe Masiero Salvarani
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  10. José Diomedes Barbosa
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Correspondence to Tatiane Teles Albernaz.

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Albernaz, T.T., Leite, R.C., Reis, J.K.P. et al. Molecular detection of bovine immunodeficiency virus in water buffaloes (Bubalus bubalis) from the Amazon region, Brazil. Trop Anim Health Prod 47, 1625–1628 (2015). https://doi.org/10.1007/s11250-015-0884-6

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  • DOI: https://doi.org/10.1007/s11250-015-0884-6

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