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Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico

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Abstract

The aim of this study was to determine the seroprevalence and presence of Chlamydia abortus in Saanen breed female goats from commercial dairy goat farms under intensive production in the municipality of Guanajuato, Mexico. Sera were collected to determine the prevalence of anti-C. abortus IgG antibodies using recombinant enzyme-linked immunosorbent assay (rELISA) and cell culture. Polymerase chain reaction (PCR) was used to prove the presence of the pathogen in swab samples collected from the vagina and rectum of selected animals. Additionally, foetal tissue samples from a sudden abortion were collected. C. abortus prevalence in female goats of commercial milking farms sampled in Guanajuato, Mexico, was 4.87 % (n = 246). Seropositive animals were found in six out of nine (66.6 %) dairy goat farms sampled, and prevalence among animals in individual farms ranged between 3.44 and 13.51 %. C. abortus was detected using PCR in spleen tissue from the aborted foetus. PCR-based detection, as well as isolation from vaginal and rectal swabs, was not possible in the present study. Isolation through cell culture was also unsuccessful from aborted foetal tissue samples. In conclusion, the results from rELISA and PCR show that C. abortus is present in dairy goat farms in the state of Guanajuato, Mexico.

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Conflict of interests

The author(s) declare no potential conflicts of interest with respect to the research, authorship and/or publication of this article.

Financial disclosure

This work was funded by the Autonomous University of the State of Mexico, No. 2330/2006U.

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Authors and Affiliations

  1. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Estado de México, México

    Eleuterio Campos-Hernández, Juan Carlos Vázquez-Chagoyán, Abdelfattah Z. M. Salem, Sandra M. López-Heydeck & Roberto Montes de Oca-Jiménez

  2. Laboratorio de Epidemiología y Zoonosis, Instituto de Investigación Científica Área Ciencias Naturales, UAGro, Chilpancingo, Mexico

    Eleuterio Campos-Hernández

  3. Departamento de Producción Agrícola y Animal, UAM-X, Mexico, Mexico

    Jorge Antonio Saltijeral-Oaxaca

  4. Facultad de Medicina Veterinaria y Zootecnia, UNAM, Mexico, Mexico

    Cristina Escalante-Ochoa

Authors
  1. Eleuterio Campos-Hernández
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  2. Juan Carlos Vázquez-Chagoyán
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  3. Abdelfattah Z. M. Salem
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  4. Jorge Antonio Saltijeral-Oaxaca
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  5. Cristina Escalante-Ochoa
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  6. Sandra M. López-Heydeck
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  7. Roberto Montes de Oca-Jiménez
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Corresponding authors

Correspondence to Abdelfattah Z. M. Salem or Roberto Montes de Oca-Jiménez.

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Campos-Hernández, E., Vázquez-Chagoyán, J.C., Salem, A.Z.M. et al. Prevalence and molecular identification of Chlamydia abortus in commercial dairy goat farms in a hot region in Mexico. Trop Anim Health Prod 46, 919–924 (2014). https://doi.org/10.1007/s11250-014-0585-6

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  • DOI: https://doi.org/10.1007/s11250-014-0585-6

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