Skip to main content
SpringerLink
Log in

Porous Poly(vinyl alcohol) Composite Membranes for Immobilization of Glucose Oxidase

  • Original Paper
  • Published:
Topics in Catalysis Aims and scope Submit manuscript

Abstract

Particle loaded porous poly(vinyl alcohol) composite membranes were selected for immobilization of glucose oxidase (GOx) for their hydrophilicity and unique interactions with amino functional groups. GOx was immobilized on the membranes by adsorption at pH values between 3.5 and 7.1. The highest adsorption loading was observed at pH 7.1 and the highest catalytic activity was observed at pH 5.1. Infrared studies showed that the highest ratio of amide I to amide II at pH 5.1 is obtained for GOx immobilized on membranes loaded with amine-functionalized micro-particles, suggesting that the conformational changes of GOx on these membranes yield to higher catalytic activity than in other supports.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. DiCosimo R, McAuliffe J, Poulose AJ, Bohlmann G (2013) Chem Soc Rev 15:6437

    Article  Google Scholar 

  2. Datta S, Christena LR, Rajaram Y (2013) 3. Biotech 1:1

    Google Scholar 

  3. Liu W, Wang L, Jiang R (2012) Top Catal 16–18:1146

    Article  Google Scholar 

  4. Bankar SB, Bule MV, Singhal RS, Ananthanarayan L (2009) Biotechnol Adv 4:489

    Article  Google Scholar 

  5. Shao Q, Qian Y, Wu P, Zhang H, Cai C (2013) Colloids Surf B 109:115

    Article  CAS  Google Scholar 

  6. Schultz N, Metreveli G, Franzreb M, Frimmel FH, Syldatk C (2008) Colloids Surf B 1:39

    Article  Google Scholar 

  7. André J, Borneman Z, Wessling M (2013) Ind Eng Chem Res 26:8635

    Article  Google Scholar 

  8. Bellusci M, Francolini I, Martinelli A, D’Ilario L, Piozzi A (2012) Biomacromolecules 3:805

    Article  Google Scholar 

  9. Guiomar AJ, Guthrie JT, Evans SD (1999) Langmuir 4:1198

    Article  Google Scholar 

  10. Carbone K, Casarci M, Varrone M (1999) J Appl Polym Sci 8:1881

    Article  Google Scholar 

  11. Gao F, Ma G (2012) Top Catal 16–18:1114

    Article  Google Scholar 

  12. Gaffney D, Cooney J, Magner E (2012) Top Catal 16–18:1101

    Article  Google Scholar 

  13. Xiao C, Ma P, Geng N (2011) Polym Adv Technol 12:2649

    Article  Google Scholar 

  14. Wang J, Yao H-B, He D, Zhang C-L, Yu S-H (2012) ACS Appl Mater Interfaces 4:1963

    Article  CAS  Google Scholar 

  15. Zhang N, Xie J, Varadan VK (2006) Smart Mater Struct 1:123

    Article  Google Scholar 

  16. Djennad MH, Benachour D, Berger H, Schomaecker R (2003) Eng Life Sci 11:446

    Article  Google Scholar 

  17. Lozinsky VI, Zubov AL, Titova EF (1997) Enzyme Microb Technol 3:182

    Article  Google Scholar 

  18. Fejerskov B, Jensen BEB, Jensen NBS, Chong S-F, Zelikin AN (2012) ACS Appl Mater Interfaces 9:4981

    Article  Google Scholar 

  19. Ren G, Xu X, Liu Q, Cheng J, Yuan X, Wu L, Wan Y (2006) React Funct Polym 12:1559

    Article  Google Scholar 

  20. Tran D, Balkus K Jr (2012) Top Catal 16–18:1057

    Article  Google Scholar 

  21. De QAAA, Passos ED, De BAS, Silva GS, Higa OZ, Vitolo M (2006) J Appl Polym Sci 2:1553

    Google Scholar 

  22. Wong F-L, Abdul-Aziz A (2008) J Chem Technol Biotechnol 1:41

    Article  Google Scholar 

  23. Kim S-G, Kim Y-I, Yun H-G, Lim G-T, Lee K-H (2003) J Appl Polym Sci 13:2884

    Article  Google Scholar 

  24. Bhattacharya M, Chaudhry S (2013) Mater Sci Eng C 33:2601

    Article  CAS  Google Scholar 

  25. Harton SE, Kumar SK, Yang H, Koga T, Hicks K, Lee H, Mijovic J, Liu M, Vallery RS, Gidley DW (2010) Macromolecules 7:3415

    Article  Google Scholar 

  26. Ha S, Wee Y, Kim J (2012) Top Catal 16–18:1181

    Article  Google Scholar 

  27. Rayalu S, Yadav R, Wanjari S, Prabhu C, Mushnoori S, Labhsetwar N, Satyanarayanan T, Kotwal S, Wate SR, Hong S-G, Kim J (2012) Top Catal 16–18:1217

    Article  Google Scholar 

  28. Silva E, Chakravartula S, Chuang SC (2012) Top Catal 7–10:580

    Article  Google Scholar 

  29. Lott JA, Turner K (1975) Clin Chem 12:1754

    Google Scholar 

  30. Ghosh Datta S, Dou X, Shibley A, Datta B (2012) Int J Biol Macromol 3:552

    Article  Google Scholar 

  31. Zhu S, Brown MF, Feller SE (2013) J Am Chem Soc 25:9391

    Article  Google Scholar 

  32. Sharma KP, Choudhury CK, Srivastava S, Davis H, Rajamohanan PR, Roy S, Kumaraswamy G (2011) J Phys Chem B 29:9059

    Article  Google Scholar 

  33. Bessbousse H, Rhlalou T, Verchère JF, Lebrun L (2008) J Memb Sci 2:249

    Article  Google Scholar 

  34. Ge L, Zhao Y-S, Mo T, Li J-R, Li P (2012) Food Control 1:188

    Article  Google Scholar 

  35. Seehuber A, Dahint R (2013) J Phys Chem B 23:6980

    Article  Google Scholar 

  36. Khodadadei F, Ghourchian H, Soltanieh M, Hosseinalipour M, Mortazavi Y (2014) Electrochim Acta 115:378

    Article  CAS  Google Scholar 

  37. Mukhopadhyay K, Phadtare S, Vinod VP, Kumar A, Rao M, Chaudhari RV, Sastry M (2003) Langmuir 9:3858

    Article  Google Scholar 

  38. Oh C, Lee J-H, Lee Y-G, Lee Y-H, Kim J-W, Kang H-H, Oh S-G (2006) Colloids Surf B 2:225

    Article  Google Scholar 

  39. Haouz A, Twist C, Zentz C, Tauc P, Alpert B (1998) Eur Biophys J 1:19

    Article  Google Scholar 

Download references

Acknowledgments

This work was supported by the FirstEnergy Advanced Energy Research Center.

Author information

Authors and Affiliations

  1. Department of Polymer Science, The University of Akron, Akron, OH, 44325-3909, USA

    Ernesto Silva Mojica & Steven S. C. Chuang

  2. Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH, 44325-3906, USA

    Mehdi Lohrasbi

Authors
  1. Ernesto Silva Mojica
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mehdi Lohrasbi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Steven S. C. Chuang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Steven S. C. Chuang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Silva Mojica, E., Lohrasbi, M. & Chuang, S.S.C. Porous Poly(vinyl alcohol) Composite Membranes for Immobilization of Glucose Oxidase. Top Catal 57, 1490–1497 (2014). https://doi.org/10.1007/s11244-014-0323-3

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11244-014-0323-3

Keywords

Search

Navigation