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Development of high frequency multiple shoot formation in Persian clover (Trifolium resupinatum L.)

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Abstract

An efficient and reliable micropropagation system for Persian clover (Trifolium resupinatum L.) was developed using different explants and media. Node, hypocotyl and cotyledonary node explants were cultured on Murashige and Skoog (MS) medium supplemented with combinations of either 6-benzyladenine (BA) and indole-3-butyric acid (IBA) or BA, Kinetin (KIN) and IBA. Direct multiple shoots developed within 6 weeks in all explants in most media tested. The best shoot multiplication capacity was obtained from cotyledonary node explants on MS medium containing 7.1 μM BA and 1 μM IBA or 14.1 μM BA and 1 μM IBA. Elongated shoots were rooted on either MS medium alone or combination with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA) and α-naphthaleneacetic acid (NAA). High rooting was achieved in half strength MS medium containing 8 μM IBA.

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Abbreviations

BA:

6-benzyladenine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KIN:

Kinetin

MS:

Murashige and Skoog (1962) medium

NAA:

α-naphthaleneacetic acid

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Authors and Affiliations

  1. Central Research Institute for Field Crops, Yenimahalle Ankara, Turkey

    Serkan Uranbey

  2. Department of Field Crops, Faculty of Agriculture, University of Ankara, Dışkapı-Ankara, Turkey, 06110

    Cafer S. Sevimay & Sebahattin Özcan

Authors
  1. Serkan Uranbey
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  2. Cafer S. Sevimay
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  3. Sebahattin Özcan
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Correspondence to Serkan Uranbey.

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Uranbey, S., Sevimay, C.S. & Özcan, S. Development of high frequency multiple shoot formation in Persian clover (Trifolium resupinatum L.). Plant Cell Tiss Organ Cult 80, 229–232 (2005). https://doi.org/10.1007/s11240-004-0568-9

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  • DOI: https://doi.org/10.1007/s11240-004-0568-9

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