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Morphological and molecular evidence for a new species of the genus Dichelyne Jägerskiöld, 1902 (Ascaridida: Cucullanidae) from marine perciform fishes in the South China Sea

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Abstract

A new cucullanid nematode, Dichelyne (Dichelyne) breviculus n. sp., collected from the intestine of the goatee croaker Dendrophysa russelii (Cuvier) (Perciformes: Sciaenidae), the burrowing goby Trypauchen vagina (Bloch & Schneider) and the tropical sand goby Acentrogobius caninus (Valenciennes) (Perciformes: Gobiidae) in the South China Sea, is described using both light and scanning electron microscopy. The new species differs from its congeners in the size of body (2.16–2.96 mm in male), the position of the excretory pore and deirids, the length of the spicules (0.90–1.32 mm, representing 32.4–51.9% of body length), the arrangement of the caudal papillae and the morphology of the tail. In addition, in order to primarily assess the validity of the new species genetically, the specimens of D. breviculus n. sp. collected from the three different hosts were also characterised using molecular methods by sequencing and analysing ribosomal [small ribosomal subunit (18S rDNA) and internal transcribed spacer (ITS)] and mitochondrial [cytochrome c oxidase subunit 1 (cox1)] target regions. The molecular analyses support the validity of the new species based on the morphological observations.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (No. 31101615), the Natural Science Foundation of Hebei Province (No. C2012205007), the China Postdoctoral Science Foundation (Nos. 2012M520593, 2014T70227), the Natural Science Foundation of Hebei Education Department (No. Y2012012), the Postdoctoral Research Projects Merit Subsidy of Human Resources and Social Security Department of Hebei Province.

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Authors and Affiliations

  1. Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Science, Hebei Normal University, Shijiazhuang, 050024, Hebei, People’s Republic of China

    Liang Li, Zhen Xu & Lu-Ping Zhang

  2. Medical College of Hebei University of Engineering, Handan, 056002, Hebei, People’s Republic of China

    Zhen Xu

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  1. Liang Li
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  2. Zhen Xu
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  3. Lu-Ping Zhang
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Correspondence to Liang Li.

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Li, L., Xu, Z. & Zhang, LP. Morphological and molecular evidence for a new species of the genus Dichelyne Jägerskiöld, 1902 (Ascaridida: Cucullanidae) from marine perciform fishes in the South China Sea. Syst Parasitol 89, 107–116 (2014). https://doi.org/10.1007/s11230-014-9516-1

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  • DOI: https://doi.org/10.1007/s11230-014-9516-1

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