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The Effect of Amino Acids as Components of Nutrient Medium on the Accumulation of Protoberberine Alkaloids in the Cell Culture of Thalictrum minus

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Abstract

We studied the effects of amino acids on the biosynthesis of protoberberine alkaloids. When 5 mM tyrosine was added to the nutrient medium, the content of alkaloids was reduced by 23% and dry weight was only 77% of the control. On the medium with 1 mM L-tryptophan, the content of alkaloids was somewhat increased (by 20%). Other amino acids (sulfur-containing L-cysteine and L-methionine, and also L-proline and L-arginine) did not affect substantially the content of alkaloids. The addition of 1 and 5 mM L-phenylalanine, which is not a primary precursor to alkaloids, induced the accumulation of alkaloids by the 17th day of the growth cycle by 40 and 140%, respectively, as compared to control treatment. The comparison of various phenylalanine concentrations showed that 7 mM phenylalanine added on the 7– 8th day induced the highest accumulation of alkaloids in the culture medium (above 1 g/l). The content of alkaloids and soluble phenolic compounds increased threefold in both the medium and cells. None of the amino acid tested enhanced biomass accumulation.

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Abbreviations

MS:

Murashige and Skoog nutrient medium

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871, Russia

    V. V. Urmantseva, O. A. Gaevskaya, T. B. Karyagina & D. I. Bairamashvili

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  1. V. V. Urmantseva
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  2. O. A. Gaevskaya
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  3. T. B. Karyagina
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  4. D. I. Bairamashvili
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__________

Translated from Fiziologiya Rastenii, Vol. 52, No. 3, 2005, pp. 438–442.

Original Russian Text Copyright © 2005 by Urmantseva, Gaevskaya, Karyagina, Bairamashvili.

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Urmantseva, V.V., Gaevskaya, O.A., Karyagina, T.B. et al. The Effect of Amino Acids as Components of Nutrient Medium on the Accumulation of Protoberberine Alkaloids in the Cell Culture of Thalictrum minus . Russ J Plant Physiol 52, 388–391 (2005). https://doi.org/10.1007/s11183-005-0058-x

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  • DOI: https://doi.org/10.1007/s11183-005-0058-x

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