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Fluorescence spectroscopy of reconstituted peridinin–chlorophyll–protein complexes

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Abstract

Peridinin–chlorophyll–proteins (PCP) were reconstituted with binary 1:1 chlorophyll (Chl) mixtures of Chl a, Chl b, [3–acetyl]-Chl a (acChl a), and studied by bulk and single-molecule fluorescence spectroscopy. The latter provides a way to distinguish in a given sample hetero-chlorophyllous complexes that contain two different Chls from homo-chlorophyllous ones containing the same Chl in both binding sites. The results are compared with those of homo-chlorophyllous PCP reconstituted with pure Chl a, Chl b, or acChl a. Relative intensities of the Chl fluorescence in hetero-chlorophyllous complexes were obtained and modeled using the Förster description of energy transfer combined with known variations of peridinin (Per)–Chl excitation transfer rates for the different Chl pigments. In the case of hetero-chlorophyllous complexes containing acChl a, the energy transfer is unidirectional in the energetically preferable direction, while it is bi-directional in the sample reconstituted with Chl a and Chl b.

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Abbreviations

Chl:

Chlorophyll

Per:

Peridinin

acChl a :

[3-Acetyl]-chlorophyll a

PCP:

Peridinin–chlorophyll–protein

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Acknowledgements

The authors thank Roger Hiller for providing purified peridinin used in the reconstitution. The work was supported by the Deutsche Forschungsgemeinschaft, Bonn (SFB 533, projects A6 and B7). S. M. acknowledges financial support from the Alexander von Humboldt Foundation.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry and Center for Nanoscience, Ludwig-Maximilian-University, Butenandtstrasse 11, 81377, Munich, Germany

    S. Mackowski, S. Wörmke, T. H. P. Brotosudarmo & C. Bräuchle

  2. Department of Biology I-Botanik, Ludwig-Maximilian-University, 80638, Munich, Germany

    T. H. P. Brotosudarmo & H. Scheer

Authors
  1. S. Mackowski
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  2. S. Wörmke
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  3. T. H. P. Brotosudarmo
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  4. H. Scheer
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  5. C. Bräuchle
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Correspondence to S. Mackowski.

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Mackowski, S., Wörmke, S., Brotosudarmo, T.H.P. et al. Fluorescence spectroscopy of reconstituted peridinin–chlorophyll–protein complexes. Photosynth Res 95, 253–260 (2008). https://doi.org/10.1007/s11120-007-9243-4

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  • DOI: https://doi.org/10.1007/s11120-007-9243-4

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