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Alteration of nitrogen metabolism in rice variety 'Nipponbare' induced by alkali stress

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Abstract

Aims

Alkali stress (AS) is an important agricultural contaminant and has complex effects on plant metabolism, specifically root physiology. The aim of this study was to test the role of nitrogen metabolism regulation in alkali tolerance of rice variety 'Nipponbare'.

Methods

In this study, the rice seedlings were subjected to salinity stress (SS) or AS. Growth, the contents of inorganic ions, NH +4 -nitrogen (free amino acids), and NO −3 -nitrogen in the stressed seedlings were then measured. The expression of some critical genes involved in nitrogen metabolism were also assayed to test their roles in the regulation of nitrogen metabolism during adaptation of rice variety 'Nipponbare' to AS.

Results

AS showed a stronger inhibiting effect on rice variety 'Nipponbare' growth than SS. AS may have more complex effects on nitrogen metabolism than SS.

Conclusions

Effects of AS on the nitrogen metabolism of rice variety 'Nipponbare' mainly comprised two mechanisms. Firstly, in roots, AS caused the reduction of NO −3 content, which caused two harmful consequences, the large downregulation of OsNR1 expression and the subsequent reduction of NH +4 production in roots. On the other hand, under AS (pH, 9.11), almost all the NH +4 was changed to NH3, which caused a severe deficiency of NH +4 surrounding the roots. Both events might cause a severe deficiency of NH +4 in roots. Under AS, the increased expression of several OsAMT family members in roots might be an adaptative response to the reduction of NH +4 content in roots or the NH +4 deficiency in rhizosphere. Also, the down-regulation of OsNADH-GOGAT and OsGS1;2 in roots might be due to NH +4 deficiency in roots. Secondly, in shoots, AS caused a larger acuumulatiuon of Na+, which possibly affected photorespiration and led to a continuous decrease of NH +4 production in shoots, and inhibited the expression of OsFd-GOGAT and OsGS2 in chloroplasts.

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Abbreviations

AMT:

Ammonium transporter

AS:

Alkali stress

FA:

Free activity

FC:

Free concentration

GDH:

Glutamate dehydrogenase

GOGAT:

Glutamate synthase

GS:

Glutamine synthetase

NR:

Nitrate reductase

NRT:

Nitrate transporter

OsAS:

Rice asparagine synthetase

P5CS:

Pyrroline-5-carboxylate

ProDH:

Proline dehydrogenase

SS:

Salinity stress

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31072078) and the Fundamental Research Funds for the Central Universities (No. 10SSXT143).

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Authors and Affiliations

  1. Key laboratory of Molecular Epigenetics of MOE, Northeast Normal University, Changchun, 130024, Jilin Province, China

    Huan Wang, Decheng Shi, Bao Liu & Chunwu Yang

  2. College of Chemistry and Biological Science, Yili Normal University, Yili, 835000, Xinjiang Municipality, China

    Jianaer Ahan

  3. Department of Agronomy, Jilin Agricultural University, Changchun, 130118, Jilin Province, China

    Zhihai Wu

Authors
  1. Huan Wang
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  2. Jianaer Ahan
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  3. Zhihai Wu
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  4. Decheng Shi
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  5. Bao Liu
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  6. Chunwu Yang
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Correspondence to Chunwu Yang.

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Responsible Editor: Timothy J. Flowers.

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Table S1

Gene-specific primers used in real time PCR analysis (DOC 52 kb)

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Wang, H., Ahan, J., Wu, Z. et al. Alteration of nitrogen metabolism in rice variety 'Nipponbare' induced by alkali stress. Plant Soil 355, 131–147 (2012). https://doi.org/10.1007/s11104-011-1086-2

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