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Heavy metal uptake by arbuscular mycorrhizas of Elsholtzia splendens and the potential for phytoremediation of contaminated soil

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Abstract

A pot culture experiment and a field experiment were carried out separately to study heavy metal (HM) uptake from soil contaminated with Cu, Zn, Pb and Cd by Elsholtzia splendens Nakai ex F. Maekawa inoculated with arbuscular mycorrhizal (AM) fungi and the potential for phytoremediation. The HM-contaminated soil in the pot experiment was collected from the field experiment site. Two AM fungal inocula, MI containing only one AM fungal strain, Glomus caledonium 90036, and M II consisting of Gigaspora margarita ZJ37, Gigaspora decipens ZJ38, Scutellospora gilmori ZJ39, Acaulospora spp. andGlomus spp., were applied to the soil under unsterilized conditions. In the pot experiment, the plants were harvested after 24 weeks of growth. Mycorrhizal colonization rate, plant dry weight (DW) and P, Cu, Zn, Pb, Cd concentrations were determined. MI-treated plants had higher mycorrhizal colonization rates than MII-treated plants. Both MI and MII increased shoot and root DW, and MII was more effective than MI. In shoots, the highest P, Cu, Zn and Pb concentrations were all observed in the plants treated with MII, while MI decreased Zn and Pb concentrations and increased P but did not alter Cu, and Cd concentrations were not affected by either of two inocula. In roots, MII increased P, Zn, Pb concentrations but did not alter Cu and Cd, and MI did not affect P, Cu, Zn, Pb, Cd concentrations. Cu, Zn, Pb, Cd uptake into shoots and roots all increased in MII-treated plants, while in MI-treated plants, Cu and Zn uptake into shoots and Cu, Zn, Pb, Cd into roots increased but Pb and Cd uptake into shoots decreased. In general, MII was more effective than MI in promoting plant growth and HM uptake. The field experiment following the pot experiment was carried out to investigate the effects of MII under field conditions. The 45-day-old nonmycorrhizal and MII-colonized seedlings of E. splendens were transplanted to HM-contaminated plots and harvested after 5 months. MII-inoculation increased shoot DW and shoot P, Cu, Zn, Pb concentrations significantly but did not alter shoot Cd concentrations, which led to higher uptake of Cu, Zn, Pb, Cd by E. splendens shoots. These results indicate that the AM fungal consortium represented by MII can benefit phytoextraction of HMs and therefore play a role in phytoremediation of HM-contaminated soils.

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Abbreviations

AM:

arbuscular mycorrhizal

HM(s):

heavy metal(s)

MD:

mycorrhizal dependency

DW:

dry weight

FW:

fresh weight

References

  • B. Bürkert A. Robson (1994) ArticleTitle 65Zn uptake in subterranean clover (Trifolium subterraneum L.) by three vesicular-arbuscular mycorrhizal fungi in a root-free sandy soil Soil Biol. Biochem. 26 1117–1124 Occurrence Handle10.1016/0038-0717(94)90133-3

    Article  Google Scholar 

  • C Del Val JM. Barea C. Azcon-Aguilar (1999) ArticleTitleAssessing the tolerance to heavy metals of arbuscular mycorrhizal fungi isolated from sewage sludge-contaminated soils Appl. Soil Ecol. 11 261–269 Occurrence Handle10.1016/S0929-1393(98)00153-X

    Article  Google Scholar 

  • G Diaz C. Azcon-Aguilar M. Honrubia (1996) ArticleTitleInfluence of arbuscular mycorrhizae on heavy metal (Zn and Pb) uptake and growth of Lygeum spartum and Anthyllis cytisoides Plant Soil 180 241–249 Occurrence Handle1:CAS:528:DyaK28XlsVOrsL8%3D

    CAS  Google Scholar 

  • TA Dueck P Visser WHO. Ernst H. Schat (1986) ArticleTitleVesicular-arbuscular mycorrhizae decrease zinc toxicity to grasses growing in zinc-polluted soil Soil Biol. Biochem. 18 331–333 Occurrence Handle10.1016/0038-0717(86)90070-2

    Article  Google Scholar 

  • AH. Fitter (1991) ArticleTitleCosts and benefits of mycorrhizas: implications for functioning under natural conditions Experientia 47 350–355 Occurrence Handle10.1007/BF01972076

    Article  Google Scholar 

  • U Galli H. Schuepp C. Brunold (1995) ArticleTitleThiols of Cu-treated maize plants inoculated with the arbuscular-mycorrhizal fungus Glomus intraradices Physiol. Plantarum. 94 247–253 Occurrence Handle1:CAS:528:DyaK2MXovFaksrg%3D

    CAS  Google Scholar 

  • A. Gaur A. Adholeya (2004) ArticleTitleProspects of arbuscular mycorrhizal fungi in phytoremediation of heavy metal contaminated soils Curr. Sci. 86 528–534 Occurrence Handle1:CAS:528:DC%2BD2cXjtVWqu7k%3D

    CAS  Google Scholar 

  • M. Giovannetti B. Mosse (1980) ArticleTitleAn evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots New Phytol. 84 489–500

    Google Scholar 

  • A Heggo A. Angle RL. Chaney (1990) ArticleTitleEffects of vesicular-arbuscular mycorrhizal fungi on heavy metal uptake by soybeans Soil Biol. Biochem. 22 865–869 Occurrence Handle10.1016/0038-0717(90)90169-Z Occurrence Handle1:CAS:528:DyaK3MXmtFSntg%3D%3D

    Article  CAS  Google Scholar 

  • I Jakobsen C. Gazey LK. Abbott (2001) ArticleTitlePhosphate transport by communities of arbuscular mycorrhizal fungi in intact soil cores New Phytol. 149 95–103 Occurrence Handle10.1046/j.1469-8137.2001.00006.x Occurrence Handle1:CAS:528:DC%2BD3MXotVSjsA%3D%3D

    Article  CAS  Google Scholar 

  • EJ. Joner I. Jakobsen (1994) ArticleTitleContribution by two arbuscular mycorrhizal fungi to P uptake by cucumber (Cucumis sativus L.) from 32P-labelled organic matter during mineralization in soil Plant Soil 163 203–209 Occurrence Handle10.1007/BF00007969 Occurrence Handle1:CAS:528:DyaK2MXislWmtrc%3D

    Article  CAS  Google Scholar 

  • EJ. Joner C. Leyval (1997) ArticleTitleUptake of 109Cd by roots and hyphae of a Glomus mosseae/Trifolium subterraneum mycorhiza from soil amended with high and low concentrations of cadmium New Phytol. 135 353–360 Occurrence Handle10.1046/j.1469-8137.1997.00633.x Occurrence Handle1:CAS:528:DyaK2sXis12itb8%3D

    Article  CAS  Google Scholar 

  • EJ. Joner C. Leyval (2001) ArticleTitleTime-course of heavy metal uptake and clover as affected by root density and different mycorrhizal inoculation regimes Biol. Fertil. Soils 33 351–357 Occurrence Handle1:CAS:528:DC%2BD3MXjtlGnsb8%3D

    CAS  Google Scholar 

  • AG Khan C Kuek TM Chaudhry CS. Khoo WJ. Hayes (2000) ArticleTitleRole of plants, mycorrhizae and phytochelators in heavy metal contaminated land remediation Chemosphere 41 197–207 Occurrence Handle10.1016/S0045-6535(99)00412-9 Occurrence Handle1:CAS:528:DC%2BD3cXivFKktr8%3D Occurrence Handle10819202

    Article  CAS  PubMed  Google Scholar 

  • RE. Koske JN. Gemma (1989) ArticleTitleA modified procedure for staining roots to detect VA mycorrhizas Mycol. Res. 92 486–505

    Google Scholar 

  • XL. Li P. Christie (2001) ArticleTitleChanges in soil solution Zn and pH and uptake of Zn by arbuscular mycorrhizal red clover in Zn contaminated soil Chemosphere 42 201–207 Occurrence Handle10.1016/S0045-6535(00)00126-0 Occurrence Handle1:CAS:528:DC%2BD3cXmvFymtL4%3D Occurrence Handle11237299

    Article  CAS  PubMed  Google Scholar 

  • JP Liao XG Lin ZH Cao YQ. Shi MH. Wong (2003) ArticleTitleInteractions between arbuscular mycorrhizae and heavy metals under sand culture experiment Chemosphere 50 847–853 Occurrence Handle10.1016/S0045-6535(02)00229-1 Occurrence Handle1:CAS:528:DC%2BD38XptlCku78%3D Occurrence Handle12688501

    Article  CAS  PubMed  Google Scholar 

  • FG. Loth W. Höfner (1995) ArticleTitleEinfluss der VA-Mykorrhiza auf die Schwermetallaufnahme von Hafer (Avena sativa L.) in Abhängigheit vom Kontaminationsgrad der Böden Z. Pflanzenernaehr. Bodenk. 158 339–345 Occurrence Handle1:CAS:528:DyaK2MXns12nsrs%3D

    CAS  Google Scholar 

  • Lu RK. (2000). Methods of Soil and Agricultural Chemistry Analysis. China Agricultural Scientech Press, Beijing. 638 pp. (in Chinese)

  • SP. McGrath FJ. Zhao E. Lombi (2001) ArticleTitlePlant and rhizosphere processes involved in phytoremediation of metal-contaminated soils Plant Soil 232 207–214 Occurrence Handle10.1023/A:1010358708525 Occurrence Handle1:CAS:528:DC%2BD3MXlsVCksLY%3D

    Article  CAS  Google Scholar 

  • AA. Meharg JWG. Cairney (1999) ArticleTitleCo-evolution of mycorrhizal symbionts and their hosts to metal contaminated environments Adv. Ecol. Res. 30 70–112

    Google Scholar 

  • JN. Pearson I. Jakobsen (1993) ArticleTitleSymbiotic exchange of carbon and phosphorus between cucumber and three arbuscular mycorrhizal fungi New Phytol. 124 481–488 Occurrence Handle1:CAS:528:DyaK2cXht12mtbs%3D

    CAS  Google Scholar 

  • N. Pearson J I. Jakobsen (1993) ArticleTitleThe relative contribution of hyphae and roots to phosphorus uptake by arbuscular mycorrhizal plants, measured by dual labeling with 32P and 33P New Phytol. 124 489–494

    Google Scholar 

  • C Plenchette JA. Fortin V. Furlan (1983) ArticleTitleGrowth response of several plants species to mycorrhiza in soil of moderate P fertility: I. mycorrhizal dependency under field conditions Plant Soil 70 191–209

    Google Scholar 

  • M Regvar K Vogel N Irgel T Wraber U Hildebrandt P. Wilde H. Bothe (2003) ArticleTitleColonization of pennycresses (Thlaspi spp.) of the Brassicaceae by arbuscular mycorrhizal fungi J. Plant Physiol. 160 615–626 Occurrence Handle10.1078/0176-1617-00988 Occurrence Handle1:CAS:528:DC%2BD3sXmtFehtb4%3D Occurrence Handle12872483

    Article  CAS  PubMed  Google Scholar 

  • NC. Schenck Y. Perez (1990) Manual for Identification of Vesicular Arbuscular Mycorrhizal Fungi, 3rd edn. Synergistic Publications University of Florida, Gainesville, Florida, USA 286

    Google Scholar 

  • SE. Smith DJ. Read (1997) Mycorrhizal Symbiosis, 2nd edn. Academic Press London 605

    Google Scholar 

  • J Song FJ Zhao YM Luo SP. McGrath H. Zhang (2004) ArticleTitleCopper uptake by Elsholtzia splendens and Silene vulgaris and assessment of copper phytoavailability in contaminated soils Environ. Pollut. 128 307–315 Occurrence Handle10.1016/j.envpol.2003.09.019 Occurrence Handle1:CAS:528:DC%2BD2cXjtFaktQ%3D%3D Occurrence Handle14720473

    Article  CAS  PubMed  Google Scholar 

  • SR Tang BM. Wilke CY. Huang (1999) ArticleTitleThe uptake of copper by plants dominantly growing on copper mining spoils along the Yangtze River, the People’s Republic of China Plant Soil 209 225–232 Occurrence Handle10.1023/A:1004599715411 Occurrence Handle1:CAS:528:DyaK1MXlt1emsrY%3D

    Article  CAS  Google Scholar 

  • C Tonin P Vandenkoornhuyse EJ Joner J. Straczek C. Leyval (2001) ArticleTitleAssessment of arbuscular mycorrhizal fungi diversity in the rhizosphere of Viola calaminaria and effect of these fungi on heavy metal uptake by clover Mycorrhiza 10 161–168 Occurrence Handle10.1007/s005720000072 Occurrence Handle1:CAS:528:DC%2BD3MXjvFyqsA%3D%3D

    Article  CAS  Google Scholar 

  • K. Turnau J. Mesjasz-Przybylowicz (2003) ArticleTitleArbuscular mycorrhiza of Berkheya coddii and other Ni-hyperaccumulating members of Asteraceae from ultramafic soils in South Africa Mycorrhiza 13 185–190 Occurrence Handle10.1007/s00572-002-0213-6 Occurrence Handle12938030

    Article  PubMed  Google Scholar 

  • A Vivas A Vörös B Biró JM Barea JM. Ruiz-Lozano R. Azcón (2003) ArticleTitleBeneficial effects of indigenous Cd-tolerant and Cd-sensitive Glomus mosseae associated with a Cd-adapted strain of Brevibacillus sp. in improving plant tolerance to Cd contamination Appl. Soil Ecol. 24 177–186 Occurrence Handle10.1016/S0929-1393(03)00088-X

    Article  Google Scholar 

  • I. Weissenhorn C. Leyval (1995) ArticleTitleRoot colonization of maize by a Cd-sensitive and a Cd-tolerant Glomus mosseae and cadmium uptake in sand culture Plant Soil 175 233–238 Occurrence Handle10.1007/BF00011359 Occurrence Handle1:CAS:528:DyaK2MXotFOltbo%3D

    Article  CAS  Google Scholar 

  • I Weissenhorn C Leyval G. Belgy J. Berthelin (1995) ArticleTitleArbuscular mycorrhizal contribution to heavy metal uptake by maize (Zea mays L.) in pot culture with contaminated soil Mycorrhiza 5 245–251 Occurrence Handle1:CAS:528:DyaK2MXnt1Cgs78%3D

    CAS  Google Scholar 

  • Xie XJ., Xu BL. (1952). Elsholtzia haichowensis: an indicator plant for copper. Acta Geol. Sin. 32, 360–367 (in Chinese)

    Google Scholar 

  • MJ Yang XE. Yang V. Römheld (2002) ArticleTitleGrowth and nutrient composition of Elsholtzia splendens Nakai under copper toxicity J. Plant Nutr. 25 1359–1375 Occurrence Handle1:CAS:528:DC%2BD38XlsFCnur0%3D

    CAS  Google Scholar 

  • Yang X E, Shi W Y, Fu C X., Yang M J 1998 Copper-hyperaccumulators of Chinese native plants: characteristics and possible use for phytoremediation. In Sustainable Agriculture for Food, Energy and Industry. Ed. NEL Bassam. pp. 484–489. James and James Publishers, London

  • YG Zhu P. Christie AS. Laidlaw (2001) ArticleTitleUptake of Zn by arbuscular mycorrhizal white clover from Zn-contaminated soil Chemosphere 42 193–199 Occurrence Handle10.1016/S0045-6535(00)00125-9 Occurrence Handle1:CAS:528:DC%2BD3cXmvFymt7c%3D Occurrence Handle11237298

    Article  CAS  PubMed  Google Scholar 

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  1. Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu Province, 210008, P.R. China

    Fayuan Wang, Xiangui Lin & Rui Yin

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  1. Fayuan Wang
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  2. Xiangui Lin
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  3. Rui Yin
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Correspondence to Fayuan Wang.

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Wang, F., Lin, X. & Yin, R. Heavy metal uptake by arbuscular mycorrhizas of Elsholtzia splendens and the potential for phytoremediation of contaminated soil. Plant Soil 269, 225–232 (2005). https://doi.org/10.1007/s11104-004-0517-8

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