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Measurements of reflectance and fluorescence spectra for nondestructive characterizing ripeness of grapevine berries

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Photosynthetica

Abstract

In vivo reflectance and fluorescence spectra from berry skins of a white (Riesling) and red (Cabernet Sauvignon) grapevine variety were measured during a ripening season with a new CMOS radiometer instrument. Classical reference measurements were also carried out for a sugar content of the berry juice [°Brix] and pigment contents (chlorophyll a and b, carotenoids, anthocyanins) from methanol extracts of the berry skin. We showed that the colours and the spectra analysed from them could be taken as an unambiguous indicator of grapevine ripening. Reflectance spectra, which were affected by the content of pigments (chlorophylls and anthocyanins), effects of surface (wax layers), and tissue structure (cell size) of the berries well correlated (R 2 = 0.89) with the °Brix measurements of the berries. The fast data acquisition of both reflectance and fluorescence spectra in one sample with our radiometer instrument made it superior over the time-consuming, traditional, and mostly destructive chemical analysis used in vine-growing management.

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Abbreviations

Anth:

anthocyanins

Car:

carotenoids

Chl:

chlorophyll

NDVI:

normalized difference vegetation index

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Authors and Affiliations

  1. Faculty of Science, Department of Physics, University of Ostrava, Chittussiho 10, CZ-710 00, Slezská Ostrava, Czech Republic

    M. Navrátil

  2. Botanical Institute, Karlsruhe Institute of Technology (KIT), Kaiserstr. 12, D-76128, Karlsruhe, Germany

    C. Buschmann

Authors
  1. M. Navrátil
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  2. C. Buschmann
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Correspondence to M. Navrátil.

Additional information

Acknowledgements: This project was supported by EU, Commission of the European Communities, 7th Framework program FP7-SME-2010-1-262011. The manuscript has been written during the stay of the first author at the Karlsruhe Institute of Technology (KIT) partially financed by the project “BioNetwork” (reg. number: CZ.1.07/2.4.00/31.0025). We would like also to acknowledge the help with measurements and data processing by Philipp Epple, Zishang Jiang, Vanessa Kunz, Marie Opálková, and Gregor Ziegler.

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Navrátil, M., Buschmann, C. Measurements of reflectance and fluorescence spectra for nondestructive characterizing ripeness of grapevine berries. Photosynthetica 54, 101–109 (2016). https://doi.org/10.1007/s11099-015-0163-9

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  • DOI: https://doi.org/10.1007/s11099-015-0163-9

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