Abstract
Purpose
Therapeutic proteins have become an integral part of health care. However, their controlled delivery remains a challenge. Protein function depends on a delicate three dimensional structure, which can be damaged during the fabrication of controlled release systems. This study presents a microgel-based controlled release system capable of high loading efficiencies, prolonged release and retention of protein function.
Methods
A new DMSO/Pluronic microemulsion served as a reaction template for the crosslinking of poly(acrylic acid) and oligo (ethylene glycol) to form microgels. Poly(acylic acid) molecular weights and microgel crosslinking densities were altered to make a series of microgels. Microgel capacity to capture and retain proteins of different sizes and isoelectric points, to control their release rate (over ~30 days) and to maintain the biofunctionality of the released proteins were evaluated.
Results
Microgels of different sizes and morphologies were synthesized. Loading efficiencies of 100% were achieved with lysozyme in all formulations. The loading efficiency of all other proteins was formulation dependent. Release of lysozyme was achieved for up to 30 days and the released lysozyme retained over 90% of its activity.
Conclusions
High loading efficiencies and prolonged release of different proteins was achieved. Furthermore, lysozyme’s functionality remained uncompromised after encapsulation and release. This work begins to lay the foundation for a broad platform for the delivery of therapeutic proteins.
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Abbreviations
- AA:
-
Acrylic acid
- A-CPA:
-
4,4’-azobis(4-cyanopentanoic acid)
- CPA-DB:
-
4-cyanopentanoic acid dithiobenzoate
- DMTMM:
-
4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride
- GPC:
-
Gel permeation chromatography
- NMM:
-
4-methylmorpholine
- OEG:
-
Oligo(ethylene glycol)
- pAA:
-
Poly(acrylic acid)
- PBS:
-
Phosphate buffered saline
- pI:
-
Isoelectric point
- RAFT:
-
Reversible addition-fragmentation chain transfer
- SEM:
-
Scanning electron microscopy
References
Carter PJ. Introduction to current and future protein therapeutics: a protein engineering perspective. Exp Cell Res. 2011;317(9):1261–9.
Vermonden T, Censi R, Hennink WE. Hydrogels for protein delivery. Chem Rev. 2012;112(5):2853–88.
Leader B, Baca QJ, Golan DE. Protein therapeutics: a summary and pharmacological classification. Nat Rev Drug Discov. 2008;7(1):21–39.
Ecker DM, Jones DJ, Levine HL. The therapeutic monoclonal antibody market. mAbs. 2015;7(1):9–14.
Manning MC, Chou DK, Murphy BM, Payne RW, Katayama DS. Stability of protein pharmaceuticals: an update. Pharm Res. 2010;27(4):544–75.
Bysell H, Månsson R, Hansson P, Malmsten M. Microgels and microcapsules in peptide and protein drug delivery. Adv Drug Deliv Rev. 2013;63(13):1172–85.
Oh JK, Drumright R, Siegwart DJ, Matyjaszewski K. The development of microgels/nanogels for drug delivery applications. Prog Polym Sci. 2008;33(4):448–77.
Vinogradov SV. Colloidal microgels in drug delivery applications. Curr Pharm Des. 2007;12(36):4703–12.
Hennink WE, van Nostrum CF. Novel crosslinking methods to design hydrogels. Adv Drug Deliv Rev. 2012;64:223–36.
Gupta P, Vermani K, Garg S. Hydrogels: from controlled release to pH-responsive drug delivery. Drug Discov Today. 2002;7(10):569–79.
Yim ES, Zhao B, Myung D, Kourtis LC, Frank CW, Carter D, et al. Biocompatibility of poly(ethylene glycol)/poly(acrylic acid) interpenetrating polymer network hydrogel particles in RAW 264.7 macrophage and MG-63 osteoblast cell lines. J Biomed Mater Res A. 2009;91(3):894–902.
De Giglio E, Cafagna D, Ricci MA, Sabbatini L, Cometa S, Ferretti C, et al. Biocompatibility of poly(acrylic Acid) thin coatings electro-synthesized onto TiAlV-based implants. J Bioact Compat Polym. 2010;25(4):374–91.
Fox M, Szoka F, Frechet J. Soluble polymer carriers for the treatment of cancer: the importance of molecular architecture. Acc Chem Res. 2009;42(8):1141–51.
Pelet J, Putnam D. An in-depth analysis of polymer-analogous conjugation using DMTMM. Bioconjug Chem. 2011;22(3):329–37.
Kunishima M, Morita J, Kawachi C, Iwasaki F, Terao K, Tani S. Esterification of carboxylic acids with alcohols by 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methyl-morpholinium chloride (DMTMM). Tetrahedron. 1999;8:1255–6.
Weiser JR, Yueh A, Putnam D. Protein release from dihydroxyacetone-based poly(carbonate ester) matrices. Acta Biomater. 2013;9(9):8245–53.
Wampler FM. Formation of diacrylic acid during acrylic acid storage. Plant/Oper Prog. 1988;7(3):183–9.
Pelet JM, Putnam D. Poly(acrylic acid) undergoes partial esterification during RAFT synthesis in methanol and interchain disulfide bridging upon NaOH treatment. Macromol Chem Phys. 2012;213:2536–40.
Rejman J, Oberle V, Zuhorn IS, Hoekstra D. Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis. Biochem J. 2004;377:159–69.
Prokov A. Intracellular delivery: fundamentals and applications. Prokop A, editor. New York: Springer; 2011.
Sharma G, Valenta DT, Altman Y, Harvey S, Xie H, Mitragotri S, et al. Polymer particle shape independently influences binding and internalization by macrophages. J Control Release. 2010;147(3):408–12.
Champion JA, Walker A, Mitragotri S. Role of particle size in phagocytosis of polymeric microspheres. Pharm Res. 2009;25(8):1815–21.
Schillemans JP, Verheyen E, Barendregt A, Hennink WE, Van Nostrum CF. Anionic and cationic dextran hydrogels for post-loading and release of proteins. J Control Release. 2011;150(3):266–71.
Zhang Y, Zhu W, Wang B, Ding J. A novel microgel and associated post-fabrication encapsulation technique of proteins. J Control Release. 2005;105(3):260–8.
Gehrke SH, Uhden LH, McBride JF. Enhanced loading and activity retention of bioactive proteins in hydrogel delivery systems. J Control Release. 1998;55(1):21–33.
Wittemann A, Azzam T, Eisenberg A. Biocompatible polymer vesicles from biamphiphilic triblock copolymers and their interaction with bovine serum albumin. Langmuir. 2007;23(4):2224–30.
Hollmann O, Czeslik C. Characterization of a planar poly(acrylic acid) brush as a materials coating for controlled protein immobilization. Langmuir. 2006;22(7):3300–5.
Cooper CL, Dubin PL, Kayitmazer AB, Turksen S. Polyelectrolyte–protein complexes. Curr Opin Colloid Interface Sci. 2005;10(1–2):52–78.
Czeslik C, Jackler G, Steitz R, von Grünberg H-H. Protein binding to like-charged polyelectrolyte brushes by counterion evaporation. J Phys Chem B. 2004;108(35):13395–402.
Henzler K, Haupt B, Lauterbach K, Wittemann A, Borisov O, Ballauff M. Adsorption of beta-lactoglobulin on spherical polyelectrolyte brushes: direct proof of counterion release by isothermal titration calorimetry. J Am Chem Soc. 2010;132(9):3159–63.
Wittemann A, Haupt B, Ballauff M. Adsorption of proteins on spherical polyelectrolyte brushes in aqueous solution. Phys Chem Chem Phys. 2003;5(8):1671–7.
Meier-Koll AA, Fleck CC, Von Grünberg HH. The counterion-release interaction. J Phys Condens Matter. 2004;16(34):6041–52.
Koutsopoulos S, Unsworth LD, Nagai Y, Zhang S. Controlled release of functional proteins through designer self-assembling peptide nanofiber hydrogel scaffold. Proc Natl Acad Sci. 2009;106(12):4623–8.
Hennink WE, De Jong SJ, Bos GW, Veldhuis TFJ, van Nostrum CF. Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Int J Pharm. 2004;277(1–2):99–104.
Hoare TR, Kohane DS. Hydrogels in drug delivery: progress and challenges. Polymer. 2008;49(8):1993–2007.
Sternberg M, Hershberger D. Separation of protein with polyacrylic acids. Biochim Biophys Acta. 1974;342(1):195–206.
Zhang C, Lillie R, Cotter J, Vaughan D. Lysozyme purification from tobacco extract by polyelectrolyte precipitation. J Chromatogr A. 2005;1069(1):107–12.
Cruise GM, Scharp DS, Hubbell JA. Characterization of permeability and network structure of interfacially photopolymerized poly (ethylene glycol) diacrylate hydrogels. Biomaterials. 1998;19:1287–94.
Thilakarathne VK, Briand VA, Kasi RM, Kumar CV. Tuning Hemoglobin—poly(acrylic acid) interactions by controlled chemical modification with triethylenetetramine. J Phys Chem B. 2012;116(42):12783–92.
Thilakarathne V, Briand VA, Zhou Y, Kasi RM, Kumar CV. Protein polymer conjugates: improving the stability of hemoglobin with poly(acrylic acid). Langmuir. 2011;27(12):7663–71.
ACKNOWLEDGMENTS AND DISCLOSURES
We would like to gratefully acknowledge the Coleman Foundation and the NSF Graduate Research Fellowship for their support (J.L.R.). The authors would like to acknowledge Dr. Lindsey Crawford for her assistance with cell culture and MTT assay. This work made use of Cornell Center for Materials Research facilities funded through NSF MRSEC DMR-1120296 and the Cornell University NMR Chemistry facility.
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Rios, J.L., Lu, G., Seo, N.E. et al. Prolonged Release of Bioactive Model Proteins from Anionic Microgels Fabricated with a New Microemulsion Approach. Pharm Res 33, 879–892 (2016). https://doi.org/10.1007/s11095-015-1834-8
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DOI: https://doi.org/10.1007/s11095-015-1834-8