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Enhancement of In Vivo Antioxidant Ability in the Brain of Rats Fed Tannin

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Abstract

The effect of the oral administration of mimosa tannin (MMT) on the rat intra-hippocampal antioxidant ability was examined. Wistar rats at the age of 6 weeks were reared for 8 weeks with the rodent diet (RD) consisting of 0.1 g/kg of MMT (RD–MMT). The antioxidant ability of rat brain was evaluated from the decay of a brain–blood-barrier permeable stable nitroxide, 3-methoxycarbonyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (PCAM) measured by the microdialysis-electron spin resonance system under a freely moving state. The decay rate of PCAM in the brain of rats fed RD–MMT was significantly larger than that of rats fed control rodent diet, which indicates the increase of the antioxidant ability in the brain of rats fed RD–MMT. In vitro study showed that MMT did not reduce PCAM directly but enhanced the reduction of PCAM by ascorbic acid. These results indicate that MMT is a potent antioxidant in vitro and in vivo.

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Acknowledgments

This work was supported by a cooperative Grant for innovative technology and advanced research in evolutional areas from the Ministry of Education, Science, Sports, and Culture of Japan.

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Authors and Affiliations

  1. Section of Obstetrics and Gynecology, Department of Reproductive and Developmental Medicine, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

    Akira Nakajima, Emiko Matsuda, Hiroshi Sameshima & Tsuyomu Ikenoue

  2. Section of Psychiatry, Department of Clinical Neuroscience, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan

    Yuto Ueda

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  1. Akira Nakajima
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  2. Yuto Ueda
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  3. Emiko Matsuda
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  4. Hiroshi Sameshima
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  5. Tsuyomu Ikenoue
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Correspondence to Akira Nakajima.

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Nakajima, A., Ueda, Y., Matsuda, E. et al. Enhancement of In Vivo Antioxidant Ability in the Brain of Rats Fed Tannin. Neurochem Res 38, 1360–1364 (2013). https://doi.org/10.1007/s11064-013-1031-2

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  • DOI: https://doi.org/10.1007/s11064-013-1031-2

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