Abstract
In the present study, we examined patterns of A-myb expression in the kainic acid (KA)-treated mouse hippocampus. Western blot analysis revealed that A-myb expression was dramatically increased in brain 3 days after KA treatment, and was sustained for more than 7 days. A-myb immunoreactivity was restricted to hippocampal neurons in control mice. Three days after KA treatment, strong A-myb immunoreactivity was observed in reactive astrocytes throughout the CA3 region. Thereafter, A-myb immunoreactive astrocytes gradually concentrated around the CA3 region in parallel with selective neuronal loss, and only a few A-myb immunoreactive astrocytes persisted in the CA3 region 14 days after KA treatment. These findings suggest that the A-myb plays a role in the reactive gliosis signaling pathway in KA-induced excitotoxic lesions.
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References
Ness SA (1996) The Myb oncoprotein: regulating a regulator. Biochim Biophys Acta 1288:F123–139
Foos G, Grimm S, Klempnauer KH (1994) The chicken A-myb protein is a transcriptional activator. Oncogene 9:2481–2488
Trauth K, Mutschler B, Jenkins NA et al (1994) Mouse A-myb encodes a trans-activator and is expressed in mitotically active cells of the developing central nervous system, adult testis and B lymphocytes. EMBO J 13:5994–6005
Golay J, Erba E, Bernasconi S et al (1994) The A-myb gene is preferentially expressed in tonsillar CD38+, CD39-, and sIgM- B lymphocytes and in Burkitt’s lymphoma cell lines. J Immunol 153:543–553
Sleeman JP (1993) Xenopus A-myb is expressed during early spermatogenesis. Oncogene 8:1931–1941
Weston K, Bishop JM (1989) Transcriptional activation by the v-myb oncogene and its cellular progenitor, c-myb. Cell 58:85–93
Sitzmann J, Noben-Trauth K, Klempnauer KH (1995) Expression of mouse c-myb during embryonic development. Oncogene 11:2273–2279
Jeon GS, Shin DH, Cho SS (2004) Induction of transcription factor c-myb expression in reactive astrocytes following intracerebroventricular kainic acid injection in mouse hippocampus. Neurosci Lett 360:13–16
Cho J, Lee SH, Seo JH et al (2002) Increased expression of phosphatase and tensin homolog in reactive astrogliosis following intracerebroventricular kainic acid injection in mouse hippocampus. Neurosci Lett 334:131–134
Laursen SE, Belknap JK (1986) Intracerebroventricular injections in mice. Some methodological refinements. J Pharmacol Methods 16:355–357
Jeon GS, Park SW, Kim DW et al (2004) Glial expression of the 90-kDa heat shock protein (HSP90) and the 94-kDa glucose-regulated protein (GRP94) following an excitotoxic lesion in the mouse hippocampus. Glia 48:250–258
Ness SA (2003) Myb protein specificity: evidence of a context-specific transcription factor code. Blood Cells Mol Dis 31:192–200
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This study was supported by grant no 21-2004-037 from the Seoul National University Hospital Research Fund, and in part by year 2006 the Second stage of BK 21 Project.
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Jeon, G.S., Byun, H.J., Park, S.K. et al. Induction of Transcription Factor A-myb Expression in Reactive Astrocytes Following an Excitotoxic Lesion in the Mouse Hippocampus. Neurochem Res 31, 1371–1374 (2006). https://doi.org/10.1007/s11064-006-9184-x
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DOI: https://doi.org/10.1007/s11064-006-9184-x