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An allograft mouse model for the study of hearing loss secondary to vestibular schwannoma growth

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Abstract

Vestibular schwannoma is a benign neoplasm arising from the Schwann cell sheath of the auditory-vestibular nerve. It most commonly affects both sides in the genetic condition Neurofibromatosis type 2, causing progressive high frequency sensorineural hearing loss. Here, we describe a microsurgical technique and stereotactic coordinates for schwannoma cell grafting in the vestibular nerve region that recapitulates local tumor growth in the cerebellopontine angle and inner auditory canal with resulting hearing loss. Tumor growth was monitored by bioluminescence and MRI in vivo imaging, and hearing assessed by auditory brainstem responses. These techniques, by potentially enabling orthotopic grafting of a variety of cell lines will allow studies on the pathogenesis of tumor-related hearing loss and preclinical drug evaluation, including hearing endpoints, for NF2-related and sporadic schwannomas.

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Acknowledgments

We thank Rosa Sierra and Erica Canal for technical support.

Funding

This study was funded by a Young Investigator Award (N-X.B.) and by the Neurofibromatosis Preclinical Consortium of the Children’s Tumor Foundation; Advocure NF2 Inc. and by the House Research Institute.

Author information

Authors and Affiliations

  1. House Research Institute, Center for Neural Tumor Research, Los Angeles, CA, USA

    Nicolas-Xavier Bonne, Fabrice Chareyre & Karo Tanaka

  2. Department of Otology and Neurotology, University Hospital of Lille, and INSERM U1008 “Controlled Drug Delivery System and Biomaterials”, University of Lille, Lille, France

    Nicolas-Xavier Bonne

  3. Department of Head and Neck Surgery, University of California Los Angeles, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    Jérémie Vitte & Marco Giovannini

  4. Jonsson Comprehensive Cancer Center, University of California Los Angeles, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    Jérémie Vitte & Marco Giovannini

  5. Department of Radiology, USC Keck School of Medicine, The Saban Research Institute of Children’s Hospital Los Angeles, Los Angeles, CA, USA

    Gevorg Karapetyan, Vazgen Khankaldyyan & Rex A. Moats

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  1. Nicolas-Xavier Bonne
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  2. Jérémie Vitte
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Correspondence to Marco Giovannini.

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Bonne, NX., Vitte, J., Chareyre, F. et al. An allograft mouse model for the study of hearing loss secondary to vestibular schwannoma growth. J Neurooncol 129, 47–56 (2016). https://doi.org/10.1007/s11060-016-2150-9

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  • DOI: https://doi.org/10.1007/s11060-016-2150-9

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