Special focus: Safety of Nanoparticles

Journal of Nanoparticle Research

, Volume 12, Issue 1, pp 47-53

Characterisation of nanoparticle size and state prior to nanotoxicological studies

  • Iker Montes-BurgosAffiliated withCentre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin
  • , Dorota WalczykAffiliated withCentre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin
  • , Patrick HoleAffiliated withNanoSight Ltd. Email author 
  • , Jonathan SmithAffiliated withNanoSight Ltd.
  • , Iseult LynchAffiliated withCentre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin
  • , Kenneth DawsonAffiliated withCentre for BioNano Interactions, School of Chemistry & Chemical Biology, University College Dublin

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Abstract

Before commencing any nanotoxicological study, it is imperative to know the state of the nanoparticles to be used and in particular their size and size distribution in the appropriate test media is particularly important. Particles satisfying standards can be commercially purchased; however, these invariably cannot be used directly and need to be dispersed into the relevant biological media. Often such changes in the environment or ionic strength, or a change in the particle concentration, results in some aggregation or a shift in the particle size distribution. Such unexpected aggregation, dissolution or plating out, if unaccounted for, can have a significant effect on the available nanoparticle dose and on interpretation of any results obtained thereafter. Here, we demonstrate the application of characterisation instrumentation that sizes nanoparticles based on their Brownian motion in suspension. Unlike classical light-scattering techniques, the nanoparticle tracking and analysis (NTA) technique allows nanoparticles to be sized in suspension on a particle-by-particle basis allowing higher resolution and therefore better understanding of aggregation than ensemble methods (such as dynamic light scattering (DLS) and differential centrifugation sedimentation (DCS)). Results will be presented from gold (standard) nanoparticles in biologically relevant media that emphasise the importance of characterisation of the nanoparticle dispersion. It will be shown how the NTA technique can be extended to multi-parameter analysis, allowing for characterization of particle size and light scattering intensity on an individual basis. This multi-parameter measurement capability allows sub-populations of nanoparticles with varying characteristics to be resolved in a complex mixture. Changes in one or more of such properties can be followed both in real time and in situ.

Keywords

Protein corona Nanoparticles Dispersion NTA DLS Nanoparticle tracking and analysis Environment EHS