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Isolation and characterization of 14 tetranucleotide microsatellite loci for the cannonball jellyfish (Stomolophus sp.) by next generation sequencing

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Abstract

The Cannonball jellyfish (Stomolophus sp.) is a species of jellyfish with high relevance in artisanal fishing. Studies of their populations do not extend beyond the morphological descriptions knowing that presents a great morphological variability. However, there are no genetic studies to determine the number of independent populations, so microsatellite markers become a suitable option. Since there are no species-specific microsatellite loci, in this paper, 14 new microsatellite loci are characterized. Microsatellite loci were isolated de novo through next generation sequencing, by two runs on Illumina MiSeq. A total of 506,771,269 base pair were obtained, from which 142,616 were microsatellite loci, and 1546 of them could design primers. We tested 14 primer pairs on 32 individuals from Bahía de La Paz, Gulf of California. We observed low genetic variation among loci (mean number of alleles per locus = 4.33, mean observed heterozygosity 0.381, mean expected heterozygosity 0.501). These loci are the first ones described for the species and will be helpful to carry out genetic diversity and population genetics studies.

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Acknowledgements

This work was funded by The University of California Institute for Mexico and the United States (UCMEXUS) collaborative grant awarded to FJGL and Michael N. Dawson (CN-13-617). We acknowledge the Mexican government for the collecting permits Secretaría de Agricultura, Ganadería, Desarrollo Rural, Pesca y Alimentación (SAGARPA) (DGOPA.07581.220708.188). TVC and LNGM are recipients of a CONACyT fellowship (290,839 and 301,916).

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Authors and Affiliations

  1. Laboratorio de Genética para la Conservación, Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, 23096, La Paz, B.C.S., México

    Leandro Nicolás Getino-Mamet, Tania Valdivia-Carrillo & Francisco Javier García-De León

  2. Centro para el Estudio de Sistemas Marinos, CESIMAR (CCT CENPAT–CONICET), Blvd. Brown 2915, U9120ACD, Puerto Madryn, Chubut, Argentina

    Leandro Nicolás Getino-Mamet

  3. School of Natural Sciences, University of California, Merced, 5200 North Lake Road, Merced, 95343, USA

    Liza Gómez Daglio

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  1. Leandro Nicolás Getino-Mamet
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  2. Tania Valdivia-Carrillo
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  3. Liza Gómez Daglio
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  4. Francisco Javier García-De León
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Correspondence to Francisco Javier García-De León.

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Getino-Mamet, L.N., Valdivia-Carrillo, T., Gómez Daglio, L. et al. Isolation and characterization of 14 tetranucleotide microsatellite loci for the cannonball jellyfish (Stomolophus sp.) by next generation sequencing. Mol Biol Rep 44, 257–260 (2017). https://doi.org/10.1007/s11033-017-4102-7

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  • DOI: https://doi.org/10.1007/s11033-017-4102-7

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