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Phylogenetic relationship of two popular edible Pleurotus in China, Bailinggu (P. eryngii var. tuoliensis) and Xingbaogu (P. eryngii), determined by ITS, RPB2 and EF1α sequences

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Abstract

The aims of this study are to assess the utility of the internal transcribed spacer (ITS) region, and partial translation elongation factor (EF1α) and RNA polymerase II (RPB2) genes, for differentiation of Bailinggu, P. eryngii, and P. nebrodensis; to reconstruct phylogenetic relationships between the three species; and to confirm the taxonomic status of Bailinggu based on ribosomal and protein-coding genes. Pairwise genetic distances between Bailinggu, P. eryngii, and related Pleurotus strains were calculated by using the p-distance model, and molecular phylogeny of these isolates was estimated based on ITS, RPB2, and EF1α using maximum parsimony and Bayesian methods. Differences in ITS, RPB2, and EF1α sequences show that Bailinggu, P. eryngii, and P. nebrodensis are distinct at the species level. Phylogenetic analyses reveal that P. eryngii is closer to P. nebrodensis than to Bailinggu. Sequence analyses of ribosomal and protein-coding genes confirm that P. eryngii var. tuoliensis is identical to Bailinggu. P. eryngii var. tuoliensis should be raised to species level or a new name should be introduced for Bailinggu after a thorough investigation into Pleurotus isolates from Ferula in Xinjiang Province. This study helps to resolve uncertainty regarding Bailinggu, P. eryngii and P. nebrodensis, improving the resource management of these strains. ITS, EF1α, and RPB2 sequences can be used to distinguish Bailinggu, P. eryngii and P. nebrodensis as three different species, and P. eryngii var. tuoliensis should be the scientific name for Bailinggu at present.

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Acknowledgments

Dr. Egon Horak is acknowledged for valuable suggestions to improve the manuscript. The research was financed by the Applied Basic Research, Science and Technology Department of Sichuan Province (Project No. 2013JY0114), National Public Welfare (Agriculture) Science and Technology Project (201503137), Sichuan Provincial Infrastructure of Microbial Resources (15010302) and Sichuan Provincial Innovation Ability Promotion Engineering (Project No. 2014LWJJ-005).

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Authors and Affiliations

  1. Soil and Fertilizer Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China

    Xiao-Lan He, Qian Li, Wei-Hong Peng, Zhong-Qian Huang & Bing-Cheng Gan

  2. Biogas Institute of Ministry of Agriculture, 4-13 Renmin Rd. South, Chengdu, 610041, China

    Bo Wu

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  1. Xiao-Lan He
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Correspondence to Bo Wu or Bing-Cheng Gan.

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He, XL., Wu, B., Li, Q. et al. Phylogenetic relationship of two popular edible Pleurotus in China, Bailinggu (P. eryngii var. tuoliensis) and Xingbaogu (P. eryngii), determined by ITS, RPB2 and EF1α sequences. Mol Biol Rep 43, 573–582 (2016). https://doi.org/10.1007/s11033-016-3982-2

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