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Genetic structure and inter-generic relationship of closed colony of laboratory rodents based on RAPD markers

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Abstract

Molecular genetic analysis was performed using random amplified polymorphic DNA (RAPD) on three commonly used laboratory bred rodent genera viz. mouse (Mus musculus), rat (Rattus norvegicus) and guinea pig (Cavia porcellus) as sampled from the breeding colony maintained at the Animal Facility, CSIR-Indian Institute of Toxicology Research, Lucknow. In this study, 60 samples, 20 from each genus, were analyzed for evaluation of genetic structure of rodent stocks based on polymorphic bands using RAPD markers. Thirty five random primers were assessed for RAPD analysis. Out of 35, only 20 primers generated a total of 56.88 % polymorphic bands among mice, rats and guinea pigs. The results revealed significantly variant and distinct fingerprint patterns specific to each of the genus. Within-genera analysis, the highest (89.0 %) amount of genetic homogeneity was observed in mice samples and the least (79.3 %) were observed in guinea pig samples. The amount of genetic homogeneity was observed very high within all genera. The average genetic diversity index observed was low (0.045) for mice and high (0.094) for guinea pigs. The inter-generic distances were maximum (0.8775) between mice and guinea pigs; and the minimum (0.5143) between rats and mice. The study proved that the RAPD markers are useful as genetic markers for assessment of genetic structure as well as inter-generic variability assessments.

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Acknowledgments

The authors are extremely grateful to the Director and staff members of animal facility of CSIR-IITR, Lucknow for providing the amenities required for carrying out the study. The financial support of this study was granted from CSIR-Empower project, New Delhi (IITR manuscript #3097).

Conflict of interest

The authors affirm no conflict of interest.

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  1. Animal Facility, CSIR-Indian Institute of Toxicology Research, M.G. Marg, Post Box No. 80, Lucknow, Uttar Pradesh, 226 001, India

    Mahadeo Kumar & Sharad Kumar

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  1. Mahadeo Kumar
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  2. Sharad Kumar
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Correspondence to Mahadeo Kumar.

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Kumar, M., Kumar, S. Genetic structure and inter-generic relationship of closed colony of laboratory rodents based on RAPD markers. Mol Biol Rep 41, 7273–7280 (2014). https://doi.org/10.1007/s11033-014-3612-9

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  • DOI: https://doi.org/10.1007/s11033-014-3612-9

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