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Adenovirus conducted connective tissue growth factor on extracellular matrix in trabecular meshwork and its role on aqueous humor outflow facility

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Abstract

Deposition of extracellular matrix (ECM) in trabecular meshwork, such as fibronectin, collagen IV, elastin. leads to increased resistance of trabecular meshwork in primary open angle glaucoma (POAG). Connective tissue growth factor (CTGF) is known to regulate the ECM deposits. In this study, we detect the effect of adenovirus conducted CTGF (Adv-CTGF) transfection on either the expression of ECM components or aqueous humor outflow facility. Adv-CTGF was used to transfect rat trabecular meshwork cells in vivo and in vitro. Aqueous humor outflow facility was test by microbeads perfusion. Protein expression of CTGF, fibronectin, and collagen IV was determined using Western blot. In the Adv-CTGF group, the outflow facility displayed a significant decrease from baseline. It appears as though the transfection with Adv-CTGF significantly affects the aqueous humor outflow pattern. A negative correlation between IOP and PEFL indicated that a decrease in the area of bead deposition corresponded to an overall decrease of outflow, leading to an elevated IOP. Adv-CTGF can enhance the expression of CTGF, fibronectin and collagen IV. CTGF is the novel target for treatment of POAG. It is necessary to further study to test inhibition of CTGF expression for treatment of POAG.

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Acknowledgments

This work was supported by the grant of nature science science foundation of China (81100659), the grant of nature science foundation of Heilongjiang province of China (No. D2007–80), Scientific foundation of education ministry of China (20092307120003), and Postdoctral foundation of China (20080430137, 200902418). Dr. Feng Wang and Dr. Shiguang Zhao work equally to this work and can be regarded as co-corresponding author.

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The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Ophthalmology, First Clinical College of Harbin Medical University, Harbin, 150001, China

    Ying Su, Jingli Cheng, Hongtao Liu & Feng Wang

  2. Department of Ophthalmology, Department of Anatomy and Neurobiology, Boston University School of Medicine, Boston, MA, 02118, USA

    Ying Su

  3. Department of Neurosurgery, First Clinical College of Harbin Medical University, Harbin, 150001, China

    Shiguang Zhao

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  1. Ying Su
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  2. Jingli Cheng
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  4. Feng Wang
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Correspondence to Feng Wang.

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Su, Y., Cheng, J., Liu, H. et al. Adenovirus conducted connective tissue growth factor on extracellular matrix in trabecular meshwork and its role on aqueous humor outflow facility. Mol Biol Rep 40, 6091–6096 (2013). https://doi.org/10.1007/s11033-013-2720-2

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  • DOI: https://doi.org/10.1007/s11033-013-2720-2

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