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A pilot study of gas chromatograph/mass spectrometry-based serum metabolic profiling of colorectal cancer after operation

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Abstract

Colorectal cancer (CRC) is mainly depended on the radical operation, the changing energy metabolism after operation reflects the extent, the magnitude, and the degree of surgical trauma. The aim of this study was to analyse the biochemical perturbation in the serum of CRC after operation and to evaluate their involvement in the progression of CRC. Gas chromatograph-mass spectrometry (GC-MS) in combination with pattern recognition techniques (Partial least squares discriminant analysis, supervised clustering analysis) was used to analyze serum metabolome in 30 CRC patients. A 34 endogenous metabolites included amino acid, fatty acid, carbohydrate and other intermediate metabolites were identified. Partial least squares discriminant analysis based on these metabolites discriminated preoperative from postoperative CRC group. Compared with preoperative CRC patients group, decreases in l-valine, 5-oxo-l-proline, 1-deoxyglucose, d-turanose, d-maltose, arachidonic acid and hexadecanoic acid levels and increases in l-tyrosine levels were observed in postoperative CRC patients group. The result demonstrated the GC-MS technique is an valuable tool for the characterization of the metabolic perturbation, and the metabolomic study will certainly benefit for monitoring the nutrition state of CRC patients, the prognosis and therapy evaluation of CRC patients after operation.

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Abbreviations

CRC:

Colorectal cancer

PLS-DA:

Partial least squares discriminant analysis

GC-MS:

Gas chromatography-mass spectrometry

TIC:

Total ion current

LMC:

Low molecular weight compounds

MVDA:

Multivariate data analysis

MSTFA:

Trimethylsilyl-trifluoroacetamide

DM:

Diabetes mellitus

HL:

Hyperlipoidemia

TMCS:

Trimethylchlorosilane

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Acknowledgments

We would like to express our deep and sincere gratitude to the Professor M. Michael Wolf (Boston University School of Medicine, USA) for his advice on this manuscript when he came to Shanghai. We thank Professor Harry Hua-Xiang Xia (Department of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR) for help suggestion in preparation of the revised manuscript. This study was financially supported by the Grants from Shanghai Science and Technology Development Fund (No. 05DJ14010), the Major Basic Research Program of Shanghai (No. 07DZ19505), and the Ministry of Science and Technology of People’s Republic of China (No. 2008CB517403).

Competing interests

We declare that we have no competing interests.

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Authors and Affiliations

  1. Department of Surgery, The Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University, 600 Yishan Road, 200233, Shanghai, China

    Yanlei Ma, Weijie Liu, Jiayuan Peng, Long Huang, Peng Zhang & Huanlong Qin

  2. Zhejiang Chinese Medical University, 310053, Hangzhou, China

    Xiaoping Zhao

  3. Pharmaceutical Information Institute, College of Pharmaceutical Sciences, Zhejiang University, 310058, Hangzhou, China

    Yiyu Cheng

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  1. Yanlei Ma
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Correspondence to Huanlong Qin.

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Authors’ contributions

Professor Huanlong Qin designed the total experiment; Dr Weijie Liu, Jiayuan Peng and Long Huang collected the colorectal cancer serum samples; Yanlei Ma, Xiaoping Zhao and Yiyu Cheng performed the entire study, meanwhile, analyzed and interpreted the acquired data; Yanlei Ma wrote the manuscript, Professor Huanlong Qin decided to submit the manuscript for publication.

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Ma, Y., Liu, W., Peng, J. et al. A pilot study of gas chromatograph/mass spectrometry-based serum metabolic profiling of colorectal cancer after operation. Mol Biol Rep 37, 1403–1411 (2010). https://doi.org/10.1007/s11033-009-9524-4

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  • DOI: https://doi.org/10.1007/s11033-009-9524-4

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