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Iron oxide labeling does not affect differentiation potential of human bone marrow mesenchymal stem cells exhibited by their differentiation into cardiac and neuronal cells

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Abstract

Mesenchymal stem cells (MSCs) have shown promising outcomes in cardiac and neuronal diseases. Efficient and noninvasive tracking of MSCs is essential to harness their therapeutic potential. Iron oxide nanoparticles (IONPs) have emerged as effective means to label stem cells and visualize them using magnetic resonance imaging (MRI). It is known that IONPs do not affect viability and cell proliferation of stem cells. However, very few studies have demonstrated differentiation potential of iron oxide-labeled MSCs and their differentiation into specific lineages that can contribute to cellular therapies. The differentiation of IONP-labeled human bone marrow mesenchymal stem cells (hBM-MSCs) into cardiac and neuronal lineages has never been studied. In this study, we have shown that IONP-labeled hBM-MSCs retain their differentiation potential to cardiac and neuronal cell lineages. We also confirmed that labeling hBM-MSCs with IONP does not affect their characteristic properties such as viability, cellular proliferation rate, surface marker profiling, and trilineage differentiation capacity. This study shows that IONP can be efficiently tracked, and its labeling does not alter stemness and differentiation potential of hBM-MSCs. Thus, the labeled hBM-MSCs can be used in clinical therapies and regenerative medicine.

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Author notes

  1. Sujata Mohanty and Krishan Gopal Jain were equally contributed

Authors and Affiliations

  1. Stem Cell Facility, Center of Excellence for Stem Cell Research, AIIMS, New Delhi, India

    Sujata Mohanty, Krishan Gopal Jain, Sushmita Bose Nandy & Anupama Kakkar

  2. Center for Biomedical Engineering, IIT Delhi, New Delhi, India

    Manoj Kumar, Harpal Singh & Alok Ray

  3. Department of Pathology, AIIMS, New Delhi, India

    Amit Kumar Dinda

  4. Department of Biology, Chemistry and Environmental Sciences, Northern New Mexico College, Espanola, NM, USA

    Sushmita Bose Nandy

Authors
  1. Sujata Mohanty
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  2. Krishan Gopal Jain
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  3. Sushmita Bose Nandy
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  4. Anupama Kakkar
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Correspondence to Sujata Mohanty.

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Mohanty, S., Jain, K.G., Nandy, S.B. et al. Iron oxide labeling does not affect differentiation potential of human bone marrow mesenchymal stem cells exhibited by their differentiation into cardiac and neuronal cells. Mol Cell Biochem 448, 17–26 (2018). https://doi.org/10.1007/s11010-018-3309-9

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  • DOI: https://doi.org/10.1007/s11010-018-3309-9

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