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miR-143 suppresses osteogenic differentiation by targeting Osterix

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Abstract

Osterix (Osx) is an essential transcription factor for osteoblast differentiation and bone formation. However, the knowledge of the regulation of Osx expression is poor. MicroRNAs (miRNAs), a class of small non-coding RNAs, play critical roles in numerous biological processes, including the proliferation, differentiation, and survival of cells and organisms. Herein, we aimed to explore the effect of miR-143 on Osx expression and osteogenic differentiation. miR-143, which was suppressor of the osteogenic differentiation of MC3T3-E1 cells, had decreased levels of expression during osteogenic differentiation. Moreover, Osx was identified to be a direct target of miR-143. Inhibition of Osx performed similar effect with miR-143 on osteogenic differentiation, while overexpression of Osx could partially reverse the suppressive effect of miR-143. Collectively, these data indicate that miR-143 is a novel regulator of Osx, and it might play an essential role in the regulation of osteogenic differentiation.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Orthopaedic Trauma, TianJin Hospital, Jiefang Road No. 406, Hexi District, Tianjin, 300211, China

    Enqi Li, Jinli Zhang, Tianxiang Yuan & Baotong Ma

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  1. Enqi Li
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  2. Jinli Zhang
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  3. Tianxiang Yuan
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  4. Baotong Ma
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Correspondence to Enqi Li.

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Li, E., Zhang, J., Yuan, T. et al. miR-143 suppresses osteogenic differentiation by targeting Osterix. Mol Cell Biochem 390, 69–74 (2014). https://doi.org/10.1007/s11010-013-1957-3

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  • DOI: https://doi.org/10.1007/s11010-013-1957-3

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