Abstract
In the present study, microcalorimetry was used to monitor the non-growth metabolism of Nitrosomonas europaea (N. europaea) cells, which could biodegrade the micropollutant (triclosan). The presence of triclosan promote non-growth metabolism and extend the life of N. europaea cells. However, at high concentration of triclosan (4 mg L−1), the acute toxicity was induced in cells. The addition of allylthiourea inhibited the non-growth metabolism of N. europaea, suggesting that ammonia monooxygenase is responsible for the biodegradation of triclosan. Scanning electron microscopy result showed the cell membrane damage induced by triclosan. It is concluded that triclosan would bind to ammonia monooxygenase on the cell membrane and cause acute toxicity. Triclosan (<1.0 mg L−1) is favor for the non-growth metabolism, while high concentration (4.0 mg L−1) is toxic to N. europaea cells.
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We gratefully acknowledge the financial support of Major Special Technological Programmes Concerning Water Pollution Control and Management (Granted No. 2012ZX07201002-3) and Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (No. QA201206).
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Qiu, S., Ma, F., Huang, X. et al. Microcalorimetric study on biodegradation kinetic of micropollutant in wastewater by ammonia-oxidizing bacteria. J Therm Anal Calorim 120, 345–349 (2015). https://doi.org/10.1007/s10973-014-4261-x
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DOI: https://doi.org/10.1007/s10973-014-4261-x