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Single Step Purification, Characterization and N-Terminal Sequences of a Mannose Specific Lectin from Mulberry Seeds

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Abstract

A mannose/glucose specific lectin have been isolated and purified from mulberry seeds by affinity chromatography on ConA Sepharose. The lectin is monomer in nature as judged by SDS-PAGE and its MW was estimated to be 22,000. The lectin is glycoprotein with neutral sugar content of 28.57%, and mannose and glucose were identified as carbohydrate. The lectin agglutinated rat red blood cells and in a hapten inhibition test, D-mannose and D-glucose was found to be inhibitor. The lectin also exhibited cytotoxic effect in brine shrimp lethality bioassay. The N-terminal sequences of the lectin upto 45-residues except the positions of 21, 39, 42 and 44 were identified. Sequence homology of the lectin is also discussed.

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Abbreviations

Con A:

Concanavalin A

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

MW:

Molecular weight

DEAE:

diethylaminoethyl

CM:

Carboxy methyl

BSA:

Bovine serum albumin

TLC:

Thin layer chromatography

ML:

Mulberry lectin

A. int.:

Artocarpus integrifolia

C. cren.:

Castanea crenata

O. sat.:

Oryza sative.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, 6205, Bangladesh

    Nurul Absar, T. Yeasmin & M. Salim Raza

  2. Department of Bimolecular Engineering, Faculty of Bioscience and Biotechnology, T I T, Yokohama, 226-8501, Japan

    S. Kumar Sarkar & F. Arisaka

Authors
  1. Nurul Absar
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  2. T. Yeasmin
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  3. M. Salim Raza
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  4. S. Kumar Sarkar
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  5. F. Arisaka
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Correspondence to Nurul Absar.

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Absar, N., Yeasmin, T., Raza, M.S. et al. Single Step Purification, Characterization and N-Terminal Sequences of a Mannose Specific Lectin from Mulberry Seeds. Protein J 24, 369–377 (2005). https://doi.org/10.1007/s10930-005-7590-6

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