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Determination of Rosuvastatin in Urine by Spectrofluorimetry After Liquid–Liquid Extraction and Derivatization in Acidic Medium

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Abstract

Statins are a class of drugs mostly used for treating hyperlipidemia, and rosuvastatin is the newest drug in the market belonging to this class. In this present work, a method was developed based on the molecular fluorescence technique, with the objective to quantify rosuvastatin in urine samples. For this purpose, the study of several parameters was made to achieve the maximum analytical signal (under reaction with sulfuric acid during 40 min). Also, a previous step to avoid matrix interference was carried out (liquid–liquid extraction). The limit of detection (LOD) and the limit of quantification (LOQ) were 0.38 and 1.28 mg L−1, respectively. Linear relationship between rosuvastatin concentration and it’s fluorescence intensity was found until 5.0 mg L−1. The proposed method was tested in several samples spiked with rosuvastatin and recovery was found in the range of 90 ± 10 %.

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References

  1. Tabas I (2002) J Clin Investig 110:583–590

    PubMed  CAS  Google Scholar 

  2. Hardman JG, Gilman AG, Limbird LE (1996) Goodman and Gilman’s. The pharmacological basis of therapeutics. McGraw-Hill, New York

    Google Scholar 

  3. The Pontifical Academy of Sciences (2010) Working group on atherosclerosis: the 21st century epidemic. Vaticano: s.n.

  4. Micallef MA, Garg ML (2009) J Nutr Biochem 20:927–939

    Article  PubMed  CAS  Google Scholar 

  5. Campo VL, Carvalho I (2007) Quím Nova 30:425–430

    Article  CAS  Google Scholar 

  6. Martin PD et al (2003) Clin Ther 25(11):2822–2835

    Article  PubMed  CAS  Google Scholar 

  7. Corsini A, Maggi FM, Catapano AL (1995) Pharmacol Res 31(1):9–27

    Article  PubMed  CAS  Google Scholar 

  8. Hull CK, Penman AD, Smith CK, Martin PD (2002) J Chromatogr B 772:219–228

    Article  CAS  Google Scholar 

  9. Oudhoff KA et al (2006) J Chromatogr B 832:191–196

    Article  CAS  Google Scholar 

  10. Trivedi RK et al (2005) J Pharm Biomed Anal 39:661–669

    Article  PubMed  CAS  Google Scholar 

  11. Krishna MV, Sankar DG (2007) E-J Chem 4(1):46–49

    Article  CAS  Google Scholar 

  12. Gupta A, Mishra P, Shah K (2009) E-J Chem 6:89–92

    Article  CAS  Google Scholar 

  13. Valeur B (2001) Molecular fluorescence: principles and applications. Wiley-VCH Verlag GmbH

  14. Bartzatt R (2001) J Pharmacol Toxicol Methods 45:247–253

    Article  PubMed  CAS  Google Scholar 

  15. Naassner M, Mergler M, Wolf K, Schuphan I (2002) J Chromatogr A 945:133–138

    Article  PubMed  CAS  Google Scholar 

  16. Wilderspin AF, Green NM (1983) Anal Biochem 132:449–455

    Article  PubMed  CAS  Google Scholar 

  17. Hoffmann C, Leroy-Dudal J, Patel S, Gallet O, Pauthe E (2008) Anal Biochem 372:62–71

    Article  PubMed  CAS  Google Scholar 

  18. Van Dongen JJM, Hooijkaas H, Comans-Bitter WM, Benne K, Van Os TM, De Josselin de Jong J (1985) J Immunol Methods 80:1–6

    Article  PubMed  Google Scholar 

  19. Suliman FEO, Al-Kindi SS, Al-Kindy SMZ, Al-Lawati HAJ (2006) J Chromatogr A 1101:179–184

    Article  PubMed  CAS  Google Scholar 

  20. Instituto Nacional de Metrologia Nomarlização e Qualidade Industrial. Orientações sobre validação de métodos de ensaios químicos. Rio de Janeiro: s.n., 2003 Vols. Vol. DOQ-CGCRE-008

  21. Instituto Nacional de Metrologia Nomarlização e Qualidade Industrial. Vocabulário Internacional de termos fundamentais e gerais de metrologia (VIM). Portaria INMETRO nº 102/88 2007. Vols. Instituto Nacional de Metrologia Nomarlização e Qualidade Industrial in Vocabulário Internacional de termos fundamentais e gerais de metrologia (VIM),, Vol. Portaria INMETRO nº 102/88 2007

  22. Nozaki O, Ohata T, Ohba Y, Moriyama H, Kato Y (1991) J Chromatogr B: Biomed Sci Appl 570:1–11

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank the Brazilian agencies Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and also the program of scholarships of Federal University of the State of Rio de Janeiro (Programa Institucional de Bolsas de Iniciação Científica da Universidade Federal Fluminense, PIBIC/UFF) for financial support and scholarships.

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  1. Departamento de Química Analítica, Universidade Federal Fluminense, Outeiro de São João Batista s/n, Centro, Niterói, RJ, 24020-141, Brazil

    Victor S. M. Braga, Taiza P. Mancilha, Ricardo J. Cassella & Wagner F. Pacheco

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  1. Victor S. M. Braga
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  2. Taiza P. Mancilha
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  3. Ricardo J. Cassella
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  4. Wagner F. Pacheco
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Correspondence to Wagner F. Pacheco.

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Braga, V.S.M., Mancilha, T.P., Cassella, R.J. et al. Determination of Rosuvastatin in Urine by Spectrofluorimetry After Liquid–Liquid Extraction and Derivatization in Acidic Medium. J Fluoresc 23, 49–55 (2013). https://doi.org/10.1007/s10895-012-1115-4

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  • DOI: https://doi.org/10.1007/s10895-012-1115-4

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